DeMartelly Victoria, Hurley Patrick, Lawrence Mathew, Redmond D Eugene, Rutherford Julienne
Comparative Primate Biology Laboratory, University of Illinois at Chicago, Chicago, IL, USA.
J Med Primatol. 2012 Jun;41(3):158-62. doi: 10.1111/j.1600-0684.2011.00534.x. Epub 2012 Feb 1.
Focus on the placenta as an agent of fetal development and offspring health outcomes is growing. Primate research facilities or zoos may collect and fix placental tissue for long-term storage, but little is known about the effects of formalin fixation on the non-human primate placenta.
We obtained 48 vervet monkey placentas from the St. Kitts Biomedical Research Foundation. We investigated via correlation coefficients and ANOVAs the effects of gestational age and original fresh weight on weight change due to fixation. We also used linear regression models to determine whether fixed tissue weight was predictive of fresh weight and gestational age.
Although the vervet monkey placenta is described as bidiscoid, 14.6% of the placentas in this sample were fused into a single mass. A decrease in weight was the most common response to formalin fixation, with the greatest degree of loss experienced by the heaviest placentas (ANOVA, F=5.99, P=0.005). Gestational age was unrelated to weight change. Those placentas that increased in weight had the lowest fresh weights. Fixed weights significantly predicted both fresh weight and gestational age (r(2) =0.78, P<0.00001; r(2) =0.76, P<0.00001, respectively).
This paper adds to a sparse literature on the vervet monkey placenta. That fixed placentas are excellent predictors of both fresh weight and gestational age suggests that banked tissue may be a valuable resource for reconstructing aspects of individual life history, although caution must be exercised given the variability of weight change as a function of original placental size.
胎盘作为胎儿发育和后代健康结果的影响因素，受到的关注日益增加。灵长类动物研究机构或动物园可能会收集并固定胎盘组织用于长期保存，但对于福尔马林固定对非人灵长类胎盘的影响知之甚少。
我们从圣基茨生物医学研究基金会获得了48个黑长尾猴胎盘。我们通过相关系数和方差分析研究了胎龄和原始鲜重在固定导致的重量变化方面的影响。我们还使用线性回归模型来确定固定组织重量是否可预测鲜重和胎龄。
尽管黑长尾猴胎盘被描述为双盘状，但该样本中14.6%的胎盘融合成了一个单一的团块。重量减轻是福尔马林固定最常见的反应，最重的胎盘重量损失程度最大（方差分析，F = 5.99，P = 0.005）。胎龄与重量变化无关。那些重量增加的胎盘鲜重最低。固定重量显著预测了鲜重和胎龄（r² = 0.78，P < 0.00001；r² = 0.76，P < 0.00001）。
本文补充了关于黑长尾猴胎盘的稀少文献。固定胎盘是鲜重和胎龄的良好预测指标，这表明保存的组织可能是重建个体生活史方面的宝贵资源，不过鉴于重量变化随原始胎盘大小的变化存在差异，必须谨慎使用。
