Kanerva R L, Lefever F R, Alden C L
Toxicol Pathol. 1983;11(2):129-31. doi: 10.1177/019262338301100204.
Male and female rats (20 of each sex) were killed, and a broad sample of organs were excised, weighed, and immersed in 10% neutral buffered formalin. Following 72 hours fixation the organs were reweighed. Comparison of fresh and fixed organ weights revealed statistically significant organ weight changes due to fixation. Although the fixation-induced organ weight changes varied in both direction and magnitude among organs and between sexes, the changes were consistent throughout samplings of each specific organ. The results of this study therefore suggest that fixed organ weights may be a valid alternative to fresh organ weights. A significantly larger data base must be generated, however, to determine the influence of fixation prior to weighing in the presence of the various pathologic tissue alterations observed in safety evaluation studies.
处死雄性和雌性大鼠（每种性别各20只），切除大量器官样本，称重后浸入10%中性缓冲福尔马林中。固定72小时后，再次对器官称重。新鲜器官重量与固定后器官重量的比较显示，固定导致器官重量发生了具有统计学意义的变化。尽管固定引起的器官重量变化在器官之间以及性别之间的方向和幅度各不相同，但在每个特定器官的采样过程中，这些变化是一致的。因此，本研究结果表明，固定后的器官重量可能是新鲜器官重量的有效替代指标。然而，必须生成一个显著更大的数据库，以确定在安全性评价研究中观察到的各种病理组织改变存在的情况下，称重前固定的影响。
