Piwat Supatcharin, Teanpaisan Rawee, Dahlén Gunnar, Thitasomakul Songchai, Douglas Charles William Ian
Common Oral Diseases and Epidemiology Research Center, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand.
J Investig Clin Dent. 2012 Feb;3(1):56-61. doi: 10.1111/j.2041-1626.2011.00098.x. Epub 2011 Sep 15.
To analyze the acid-producing and growth abilities of different oral Lactobacillus species.
Thirty-nine oral clinical strains and type strains of Lactobacillus, representing nine species, including Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus mucosae, Lactobacillus oris, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus salivarius, and Lactobacillus vaginalis were studied. Anaerobically-grown bacterial cells were inoculated overnight in de Man, Rogosa, and Sharpe broth containing 2% glucose (pH 7.0). Acid production and growth were measured at 0, 1.5, 3, 5, 7, and 24 h.
Lactobacillus salivarius, Lactobacillus rhamnosus, Lactobacillus casei/paracasei, and Lactobacillus plantarum grew rapidly and reached an optical density higher than other species. They also produced more acid than the others. Lactobacillus vaginalis showed the lowest rate of growth and acid production. These findings demonstrated that the different species of Lactobacillus showed different abilities to generate acid, allowing the species to be categorized into three groups: strongly, moderately, and weakly acidogenic.
There was variation in acid production and growth between the Lactobacillus species. The strongest acid producers were Lactobacillus salivarius, Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus casei/paracasei, respectively. It seems possible that these species might play a more important role in caries development than the others.
分析不同口腔乳酸杆菌属菌种的产酸能力和生长能力。
研究了39株口腔临床分离株和乳酸杆菌属标准菌株，它们代表9个菌种，包括干酪乳杆菌/副干酪乳杆菌、发酵乳杆菌、加氏乳杆菌、黏液乳杆菌、口腔乳杆菌、植物乳杆菌、鼠李糖乳杆菌、唾液乳杆菌和阴道乳杆菌。将厌氧培养的细菌细胞接种于含有2%葡萄糖（pH 7.0）的德氏、罗氏和夏普肉汤中过夜培养。在0、1.5、3、5、7和24小时测量产酸量和生长情况。
唾液乳杆菌、鼠李糖乳杆菌、干酪乳杆菌/副干酪乳杆菌和植物乳杆菌生长迅速，其光密度高于其他菌种。它们产生的酸也比其他菌种多。阴道乳杆菌的生长速率和产酸量最低。这些发现表明，不同的乳酸杆菌属菌种产酸能力不同，可分为三组：产酸强、产酸中等和产酸弱。
乳酸杆菌属菌种之间产酸和生长存在差异。最强的产酸菌分别是唾液乳杆菌、鼠李糖乳杆菌、植物乳杆菌和干酪乳杆菌/副干酪乳杆菌。这些菌种在龋齿发展中可能比其他菌种发挥更重要的作用。
