反相高效液相色谱法测定开口箭根茎中的两种皂苷
[Determination of two saponins in Tupistra chinensis rhizomes by RP-HPLC].
作者信息
Zhou Yuan, Zou Kun, Yu Ling-ling, Qin Shuang-jiu, Xu Lan-lan, Liu Chuang
机构信息
Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Sciences, China Three Gorges University, Yichang 443002, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(22):2647-9.
OBJECTIVE
To develog an HPLC method determination of two new saponins in the dried rhizomes of Tupistra chinensis.
METHOD
The analysis was performed on YMC-Pack ODS-AQ (4.6 mm x 250 mm, 5 microm) eluted with of acetonitrile and waterin gradient mode. The conentration of acetonitrile in the mobile phase changes from 10% to 100% within 85 minutes. The detection wavelength was set at 203 nm. The flow rate was 1.00 mL x min(-1) and column temperature was set at 30 degrees C.
RESULT
The linear relationships of two authentic saponins were determined within the range from 7.55 microg to 60.40 microg (r=0.9996) and 8.00 microg to 48.00 microg (r=0.9996), respectively. Using the method above, the contents of two saponins were determined as 0.261% and 0.242%, with the recoveries as 98.6% and 98.3% with RSD 1.7% and 1.7%, respectively.
CONCLUSION
A convenient and reliable method was developed to determine the content of two saponins in the dried rhizomes of Tupistra chinensis.
目的
建立高效液相色谱法测定开口箭干燥根茎中两种新皂苷的含量。
方法
采用YMC-Pack ODS-AQ（4.6 mm×250 mm，5μm）色谱柱，以乙腈和水为流动相进行梯度洗脱。流动相中乙腈的浓度在85分钟内从10%变化至100%。检测波长设定为203 nm。流速为1.00 mL·min⁻¹，柱温设定为30℃。
结果
两种对照皂苷分别在7.55μg至60.40μg（r = 0.9996）和8.00μg至48.00μg（r = 0.9996）范围内呈线性关系。采用上述方法，两种皂苷的含量分别测定为0.261%和0.242%，回收率分别为98.6%和98.3%，相对标准偏差分别为1.7%和1.7%。
结论
建立了一种简便可靠的方法来测定开口箭干燥根茎中两种皂苷的含量。
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