Nayila Iffat, Sarwar Muhammad, Hameed Saima, Iqbal Aasma, Sharif Sumaira
Department of Pharmacy, The University of Lahore Sargodha Campus Sargodha Pakistan
Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan
Nanoscale Adv. 2025 Jul 31. doi: 10.1039/d5na00341e.
As natural chemicals may have fewer side effects than conventional medicines, their usage in cancer treatment is becoming more popular. Nanoformulations have been the focus of recent developments to increase the anticancer potential of phytocompounds or pure isolated components. To evaluate the anticancer potential of silver nitrate nanoparticles (AgNPs), essential oil (EO) from was extracted, a terpene compound was isolated and incensole acetate (IA)-mediated silver nanoparticles (IA-AgNPs) were synthesized. The anticancer potential of biosynthesized IA-AgNPs was assessed using a rat mammary tumor model induced by 9,10-dimethylbenz[]anthracene (DMBA). Scanning electron microscopy (SEM), ultraviolet (UV) and visible spectroscopy, size determination, and dynamic light scattering were used to characterize the synthesized nanoparticles (NPs). To ascertain the bioactive potential of the prepared IA-AgNPs, tests for cytotoxicity, antioxidant activity, stress marker analysis, and histopathology were performed. The study's findings showed that among DMBA-induced rats, IA-AgNPs markedly improved some oxidative stress enzyme levels, and upregulated gene expression. IA-AgNPs demonstrated effective cytotoxicity against MDA-MB-231 cancer cell lines. The anticancer potential was observed by improved levels of antioxidant enzymes. Significant improvements in cancer-induced tissues were found by histopathological investigation, validating the IA-AgNPs' therapeutic effectiveness. In conclusion, the results indicate that gene expression in the DMBA-induced breast cancer (BC) model is altered by biosynthesized silver nitrate NPs. The combined findings highlight IA-AgNPs' promise as a cutting-edge, targeted nanotherapeutic strategy with improved efficacy in treating cancer parameters compared to traditional therapies.
由于天然化学物质可能比传统药物具有更少的副作用，它们在癌症治疗中的应用越来越普遍。纳米制剂一直是近期开发的重点，以提高植物化合物或纯分离成分的抗癌潜力。为了评估硝酸银纳米颗粒（AgNPs）的抗癌潜力，提取了来自[具体来源未提及]的精油（EO），分离出一种萜类化合物，并合成了乙酸沉香酯（IA）介导的银纳米颗粒（IA-AgNPs）。使用由9,10-二甲基苯并[a]蒽（DMBA）诱导的大鼠乳腺肿瘤模型评估生物合成的IA-AgNPs的抗癌潜力。使用扫描电子显微镜（SEM）、紫外（UV）和可见光谱、尺寸测定以及动态光散射来表征合成的纳米颗粒（NPs）。为了确定所制备的IA-AgNPs的生物活性潜力，进行了细胞毒性、抗氧化活性、应激标志物分析和组织病理学测试。研究结果表明，在DMBA诱导的大鼠中，IA-AgNPs显著提高了一些氧化应激酶水平，并上调了[具体基因未提及]基因表达。IA-AgNPs对MDA-MB-231癌细胞系表现出有效的细胞毒性。通过抗氧化酶水平的提高观察到了抗癌潜力。组织病理学研究发现癌症诱导组织有显著改善，证实了IA-AgNPs的治疗效果。总之，结果表明生物合成的硝酸银纳米颗粒改变了DMBA诱导的乳腺癌（BC）模型中的[具体基因未提及]基因表达。综合研究结果突出了IA-AgNPs作为一种前沿的靶向纳米治疗策略的前景，与传统疗法相比，其在治疗癌症参数方面具有更高的疗效。
