两株临床分离肠杆菌对阿普拉霉素的耐药性：3-N-乙酰基转移酶IV的检测

Resistance to apramycin in two enterobacterial clinical isolates: detection of a 3-N-acetyltransferase IV.

作者信息

Gómez-Lus R, Rivera M J, Gómez-Lus M L, Gil J, Gómez-Lus S, Castillo J, Goñi P, Madero P, Rubio M C

机构信息

Department of Microbiology, Faculty of Medicine, University of Zaragoza, Spain.

出版信息

J Chemother. 1990 Aug;2(4):241-3. doi: 10.1080/1120009x.1990.11739023.

DOI:10.1080/1120009x.1990.11739023

PMID:2230907

Abstract

Considering the possible role of farm animals in the contamination of human consumers by plasmid-mediated apramycin-resistant enterobacteria strains, this type of resistance should be tested more systematically in human isolates. Very recently we isolated in Zaragoza one apramycin-resistant Escheria coli strain obtained from the blood of a hospitalized patient; this clinical isolate produced a plasmid-mediated 3-N-aminoglycoside acetyltransferase IV. We describe also the isolation in Madrid of one multiresistant Klebsiella pneumoniae clinical strain. This isolate harbored a single plasmid and carried determinants for apramycin, gentamicin, tobramycin, hygromycin B, streptomycin, and ampicillin, which could be transferred en bloc to E. coli K-12 J62. Extracts from donor and transconjugant strains carrying pUZ6776 plasmid produce acetyltransferase activity AAC(3)-IV and double phosphotransferase activity (HPH and APH(3'')).

摘要

考虑到家畜在质粒介导的对阿普拉霉素耐药的肠杆菌菌株污染人类消费者方面可能发挥的作用，这种耐药性应在人类分离株中进行更系统的检测。最近我们在萨拉戈萨从一名住院患者的血液中分离出一株对阿普拉霉素耐药的大肠杆菌菌株；该临床分离株产生了一种质粒介导的3-N-氨基糖苷乙酰转移酶IV。我们还描述了在马德里分离出一株多重耐药的肺炎克雷伯菌临床菌株。该分离株携带一个单一质粒，并携带对阿普拉霉素、庆大霉素、妥布霉素、潮霉素B、链霉素和氨苄青霉素的决定簇，这些决定簇可以整体转移到大肠杆菌K-12 J62。携带pUZ6776质粒的供体菌株和转接合子菌株的提取物产生乙酰转移酶活性AAC(3)-IV和双磷酸转移酶活性（HPH和APH(3'')）。