Hunter J E, Bennett M, Hart C A, Shelley J C, Walton J R
Department of Veterinary Clinical Science, University of Liverpool.
Epidemiol Infect. 1994 Jun;112(3):473-80. doi: 10.1017/s0950268800051177.
Escherichia coli serotype O147:K89:K88a,c was found to be associated with outbreaks of diarrhoea in preweaner pigs of up to 4 weeks of age on a pig unit. Resistance to apramycin, gentamicin, netilmicin, tobramycin and other antibiotics was associated with conjugative plasmids of approximately 62 kb. The presence of a gene which encoded for the aminoglycoside acetyltransferase enzyme AAC(3)IV was confirmed by DNA hybridization. Samples collected during the following 12 months revealed widespread dissemination of these resistance plasmids in non-serotypable, non-haemolytic E. coli throughout the farm. Apramycin-resistant E. coli were also isolated from a stockman and it appeared from plasmid profile analysis and antibiotic sensitivity testing that the human isolates carried the same plasmid as that carried by the porcine E. coli. Klebsiella pneumoniae, with a slightly smaller conjugative plasmid and similar resistance pattern, was isolated from the stockman's wife.
在一个养猪场中，发现大肠杆菌血清型O147:K89:K88a,c与4周龄以下断奶前仔猪的腹泻暴发有关。对阿普拉霉素、庆大霉素、奈替米星、妥布霉素和其他抗生素的耐药性与大约62 kb的接合质粒有关。通过DNA杂交证实了编码氨基糖苷乙酰转移酶AAC(3)IV的基因的存在。在随后的12个月中采集的样本显示，这些耐药质粒在整个农场的不可分型、非溶血性大肠杆菌中广泛传播。还从一名饲养员身上分离出了耐阿普拉霉素的大肠杆菌，从质粒图谱分析和抗生素敏感性测试来看，人源分离株携带的质粒与猪源大肠杆菌携带的质粒相同。从饲养员的妻子身上分离出了肺炎克雷伯菌，其接合质粒略小，耐药模式相似。
