Kogure Morihiro, Mimura Naomi, Ikemoto Hideshi, Ishikawa Shintaro, Nakanishi-Ueda Takako, Sunagawa Masataka, Hisamitsu Tadashi
Department of Physiology, Showa University, School of Medicine, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
J Acupunct Meridian Stud. 2012 Feb;5(1):29-33. doi: 10.1016/j.jams.2011.11.004. Epub 2011 Dec 13.
The purpose of this study was to compare the effectiveness of moxibustion (MOX) treatment at the GV4 and CV12 acupoints, and to determine the correlations between MOX treatment and interleukin (IL)-6 and corticosterone levels in a collagen-induced arthritis (CIA) mouse model. CIA mice were immunized twice intradermally over a 3-week interval with bovine type II collagen. After the second immunization (day 21), MOX was applied to the mouse equivalent of the GV4 and CV12 acupoints with a 1mg moxa cone five times/day. Clinical symptoms of CIA were observed three times/week until day 35. The concentrations of IL-6 and corticosterone in the blood samples were measured by immunoassay kits. At day 35, the incidence of CIA was significantly decreased in mice treated with MOX at the GV4 acupoint (78%, n=23, p<0.05), compared to untreated CIA mice (100%) and mice treated with MOX at the CV12 acupoint (100%). IL-6 and corticosterone levels were significantly increased by immunization. IL-6 levels significantly decreased in mice treated with MOX at the GV4 acupoint. These results suggest that MOX treatment suppressed CIA at the GV4 acupoint, not at the CV12 acupoint, possibly through inhibition of IL-6 production.
本研究的目的是比较艾灸GV4和CV12穴位的疗效，并确定在胶原诱导的关节炎（CIA）小鼠模型中艾灸治疗与白细胞介素（IL）-6和皮质酮水平之间的相关性。将CIA小鼠在3周的间隔内分两次皮内注射牛II型胶原进行免疫。第二次免疫后（第21天），用1mg艾炷对相当于小鼠GV4和CV12的穴位进行艾灸，每天5次。每周观察3次CIA的临床症状，直至第35天。用免疫分析试剂盒测定血样中IL-6和皮质酮的浓度。在第35天，与未治疗的CIA小鼠（100%）和CV12穴位艾灸治疗的小鼠（100%）相比，GV4穴位艾灸治疗的小鼠CIA发病率显著降低（78%，n=23，p<0.05）。免疫后IL-6和皮质酮水平显著升高。GV4穴位艾灸治疗的小鼠IL-6水平显著降低。这些结果表明，艾灸治疗在GV4穴位而非CV12穴位抑制了CIA，可能是通过抑制IL-6的产生。
