Umezawa M, Hanada K, Naiki H, Chen W H, Hosokawa M, Hosono M, Hosokawa T, Takeda T
Department of Senescence Biology, Faculty of Medicine, Kyoto University, Japan.
J Nutr. 1990 Nov;120(11):1393-400. doi: 10.1093/jn/120.11.1393.
The effects of age and dietary restriction on immune response were investigated using an animal model of accelerated senescence (senescence accelerated mouse, SAM). The experimental groups consisted of control (ad libitum fed) and restricted groups (fed 60% of energy intake of the controls). Spleen weight and total number of splenic cells were significantly lower in the food-restricted group at 8 mo of age. Percentages of T (Thy-1.1+) and B (surface Ig+) cells in the splenic cells were not significantly different between the two groups. The number of direct hemolytic plaque-forming cells per 10(6) spleen cells 4 d following immunization with sheep red blood cells and dinitrophenyl-Ficoll was significantly greater in the 8-mo-old mice in the food-restricted group than in the control group. In the latter group, antibody responses Progressively decreased with age. Mitogen responses to concanavalin A and lipopolysaccharide were maintained in the food-restricted group but were depressed in the control group at 8 mo. In addition, though autoantibody to single-stranded DNA increased in the control group with advancing age, there was a steady decrease in the food-restricted group until 8 mo. Serum immunoglobulin (IgA and IgM) concentrations were significantly lower in the food-restricted group than in controls at 8 mo of age. Therefore, our results suggest that when senescence accelerated mice are subjected to food restriction, there may be a modulatory effect on the immune dysfunction associated with advancing age.
利用加速衰老动物模型（衰老加速小鼠，SAM）研究了年龄和饮食限制对免疫反应的影响。实验组包括对照组（自由采食）和限制组（摄入能量为对照组的60%）。8月龄时，食物限制组的脾脏重量和脾细胞总数显著低于对照组。两组脾细胞中T细胞（Thy-1.1+）和B细胞（表面Ig+）的百分比无显著差异。用绵羊红细胞和二硝基苯基 - 菲可（dinitrophenyl-Ficoll）免疫4天后，每10(6)个脾细胞中直接溶血空斑形成细胞的数量在食物限制组的8月龄小鼠中显著多于对照组。在对照组中，抗体反应随年龄增长逐渐降低。食物限制组对刀豆蛋白A和脂多糖的促有丝分裂反应得以维持，但对照组在8月龄时受到抑制。此外，虽然对照组中抗单链DNA自身抗体随年龄增长而增加，但食物限制组在8月龄前一直稳定下降。8月龄时，食物限制组的血清免疫球蛋白（IgA和IgM）浓度显著低于对照组。因此，我们的结果表明，当衰老加速小鼠接受食物限制时，可能对与衰老相关的免疫功能障碍有调节作用。
