Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
Curr Microbiol. 2012 May;64(5):418-26. doi: 10.1007/s00284-012-0091-x.
To search for candidate control agents against Aeromonas salmonicida subsp. salmonicida infections in aquaculture, one bacteriophage (phage), designated as PAS-1, was isolated from the sediment samples of the rainbow trout (Oncorhynchus mykiss) culture farm in Korea. The PAS-1 was morphologically classified as Myoviridae and possessed approximately 48 kb of double-strand genomic DNA. The phage showed broad host ranges to other subspecies of A. salmonicida as well as A. salmonicida subsp. salmonicida including antibiotic-resistant strains. Its latent period and burst size were estimated to be approximately 40 min and 116.7 PFU/cell, respectively. Furthermore, genomic and structural proteomic analysis of PAS-1 revealed that the phage was closely related to other Myoviridae phages infecting enterobacteria or Aeromonas species. The bacteriolytic activity of phage PAS-1 was evaluated using three subspecies of A. salmonicida strain at different doses of multiplicity of infection, and the results proved to be efficient for the reduction of bacterial growth. Based on these results, PAS-1 could be considered as a novel Aeromonas phage and might have potentiality to reduce the impacts of A. salmonicida infections in aquaculture.
为了在水产养殖中寻找针对嗜水气单胞菌亚种感染的候选控制剂，从韩国虹鳟（Oncorhynchus mykiss）养殖场的沉积物样本中分离出了一种噬菌体（噬菌体），命名为 PAS-1。PAS-1 在形态上被归类为肌尾噬菌体科，具有约 48 kb 的双链基因组 DNA。噬菌体对其他嗜水气单胞菌亚种以及包括抗生素抗性菌株在内的嗜水气单胞菌亚种具有广泛的宿主范围。其潜伏期和爆发大小分别估计约为 40 分钟和 116.7 PFU/细胞。此外，PAS-1 的基因组和结构蛋白质组分析表明，该噬菌体与感染肠杆菌或气单胞菌属的其他肌尾噬菌体密切相关。噬菌体 PAS-1 的溶菌活性在不同感染复数剂量的三种嗜水气单胞菌亚种菌株中进行了评估，结果证明对减少细菌生长有效。基于这些结果，PAS-1 可以被认为是一种新型的气单胞菌噬菌体，并且有可能减少水产养殖中气单胞菌感染的影响。
