Sikora Per, Chawade Aakash, Larsson Mikael, Olsson Johanna, Olsson Olof
Department of Plant and Environmental Sciences, Göteborg University, 40530 Göteborg, Sweden.
Int J Plant Genomics. 2011;2011:314829. doi: 10.1155/2011/314829. Epub 2012 Jan 22.
Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding. Both genetic (Targeting Induced Local Lesions in Genomes; TILLING) and phenotypic screens are evaluated. Finally, different ways of bridging the gap between genotype and phenotype are discussed.
随着高分辨率分子和生化技术的最新发展，植物诱变正迅速走向成熟。通过将诱变群体的高度变异性与新颖的筛选方法相结合，现在正在分子水平上开发和表征那些几乎不可能通过传统育种鉴定的性状。本文全面概述了当今分子育种领域研究人员可用的各种技术和工作流程，以及这些工具如何补充传统育种中已使用的工具。对遗传筛选（基因组靶向诱导局部损伤；TILLING）和表型筛选都进行了评估。最后，讨论了弥合基因型和表型之间差距的不同方法。
