Sikander Mohammad, Malik Shabnam, Yadav Deepak, Biswas Sahabuddin, Katare Deepshikha Pande, Jain Swatantar Kumar
Department of Biotechnology, F/o Science, Hamdard University, New Delhi, India.
Asian Pac J Cancer Prev. 2011;12(10):2513-6.
Dietary flavonoids have attracted attention as chemopreventive agents. Chalcones are abundantly present in nature starting from ferns to higher plants. Chemically 1,3-diphenyl-2-propen-1-ones, these are often cytotoxic in vitro. The cellular defense system (including glutathione, glutathione-related enzymes, and antioxidant and redox enzymes) plays a crucial role in cell survival and growth in aerobic organisms. In the present study, we aimed to evaluate the modulatory effect of trans-chalcone on protection from oxidative stress caused by hydrogen peroxide (H2O2) in hepatocellular carcinoma (HepG2) cells. Cell growth was evaluated by the 3-(4,5-dimethyl thiazol-2- yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Sub-toxic concentrations of compound (20 uM) increased cell survival and a decreased lipid peroxidation. The drug also decreased the H2O2 induction of glutathione related enzymes. Our results support the efficacy of trans-chalcone in offering protection against oxidative stress.
膳食类黄酮作为化学预防剂已引起关注。查耳酮广泛存在于自然界，从蕨类植物到高等植物均有。从化学结构上讲，它们是1,3 - 二苯基 - 2 - 丙烯 - 1 - 酮，通常在体外具有细胞毒性。细胞防御系统（包括谷胱甘肽、谷胱甘肽相关酶以及抗氧化和氧化还原酶）在需氧生物的细胞存活和生长中起着至关重要的作用。在本研究中，我们旨在评估反式查耳酮对肝癌（HepG2）细胞中过氧化氢（H2O2）所致氧化应激的保护调节作用。通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）法评估细胞生长。化合物的亚毒性浓度（20 μM）可提高细胞存活率并降低脂质过氧化。该药物还可降低H2O2诱导的谷胱甘肽相关酶。我们的结果支持反式查耳酮在提供抗氧化应激保护方面的功效。
