Guizani Nejib, Waly Mostafa I
Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34 Al- Khod, Postal Code 123, Oman.
Pak J Biol Sci. 2012 Nov 15;15(22):1069-74. doi: 10.3923/pjbs.2012.1069.1074.
Hydrogen peroxide is an oxidative stress agent that is associated with depletion of intracellular glutathione and inhibition of antioxidant enzymes in different cell lines. Consumption of antioxidant-rich foods reduces cellular oxidative stress and its related health problems. This study aimed to assess the antioxidant properties of mushroom, Agaricus bisporous cultivar extract, against hydrogen peroxide induced oxidative stress in cultured human hepatic (HepG2) and neuronal (SH-SY5Y) cells. In this study, hydrogen peroxide caused significant oxidative stress in HepG2 and SH-SY5Y cells as demonstrated by glutathione depletion, impairment of total antioxidant capacity and inhibition of antioxidant enzymes (glutathione peroxidase, catalase and superoxide dismutase). Agaricusbisporous extract ameliorated the observed hydrogen peroxide-induced oxidative cellular insult as indicated by restoring the activity of glutathione and the assayed antioxidant enzymes to control levels. The results suggest that mushroom extract as antioxidant properties and protects against the oxidative stress induced by hydrogen peroxide-in cultured human hepatic and neuronal cells.
过氧化氢是一种氧化应激剂，与不同细胞系中细胞内谷胱甘肽的消耗和抗氧化酶的抑制有关。食用富含抗氧化剂的食物可降低细胞氧化应激及其相关的健康问题。本研究旨在评估双孢蘑菇提取物对过氧化氢诱导的培养人肝细胞（HepG2）和神经元细胞（SH-SY5Y）氧化应激的抗氧化特性。在本研究中，过氧化氢在HepG2和SH-SY5Y细胞中引起了显著的氧化应激，表现为谷胱甘肽消耗、总抗氧化能力受损以及抗氧化酶（谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶）的抑制。双孢蘑菇提取物改善了观察到的过氧化氢诱导的氧化细胞损伤，这表现为谷胱甘肽和所检测的抗氧化酶的活性恢复到对照水平。结果表明，蘑菇提取物具有抗氧化特性，并能保护培养的人肝细胞和神经元细胞免受过氧化氢诱导的氧化应激。
