Key Laboratory of the Ministry of Education, Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, China.
Planta Med. 2012 Apr;78(6):622-5. doi: 10.1055/s-0031-1298224. Epub 2012 Feb 9.
Three new cycloartenol triterpene saponins, 3 β,16 α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24 R,25-epoxy-cycloartane-7-ene 3- O- β-D-galactopyranoside ( 1), 24- O-hydroxy-7,8-didehydrohydroshengmanol 3- O- β-D-galactopyranoside ( 2), and 24-epi-24- O-hydroxy-7,8-didehydrohydroshengmanol 3- O- β-D-galactopyranoside ( 3), were isolated from the ethyl acetate soluble fraction of Cimicifuga simplex. Their structures were established by detailed spectroscopic analysis, including extensive 2D-NMR data. This is the first time that a 16,22-cyclo type glycosidesaponin from aCimicifuga species was reported. The immunosuppressive activities of the new compounds were evaluated by a ConA-stimulated T splenocyte proliferation assay in vitro.
从升麻乙酸乙酯可溶部分中分离得到三种新的环阿尔廷醇三萜皂苷，分别为 3β,16α-二羟基-12-乙酰氧基-16,22-环-23-酮-24R,25-环氧-环阿尔廷烷-7-烯 3-O-β-D-半乳糖苷（1）、24-O-羟基-7,8-去氢升麻醇 3-O-β-D-半乳糖苷（2）和 24-表-24-O-羟基-7,8-去氢升麻醇 3-O-β-D-半乳糖苷（3）。通过详细的光谱分析，包括广泛的 2D-NMR 数据，确定了它们的结构。这是首次从苍术属植物中分离得到 16,22-环型糖苷皂苷。通过体外 ConA 刺激 T 脾淋巴细胞增殖试验评价了新化合物的免疫抑制活性。
