升麻地上部分新环羊毛甾烷三萜双糖苷的免疫抑制作用

Immunosuppressive effects of new cyclolanostane triterpene diglycosides from the aerial part of Cimicifuga foetida.

作者信息

Pan Rui-Le, Chen Di-Hua, Si Jian-Yong, Zhao Xiao-Hong, Li Zhan, Cao Li

机构信息

Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

出版信息

Arch Pharm Res. 2009 Feb;32(2):185-90. doi: 10.1007/s12272-009-1133-1. Epub 2009 Mar 13.

DOI:10.1007/s12272-009-1133-1

PMID:19280146

Abstract

Two new cyclolanostane diglycosides, cimifoetiside A (1) and cimifoetiside B (2), were isolated from an 80% ethanolic extract of the aerial part of Cimicifuga foetida L. (Ranuculaceae). Using spectral data and chemical analysis, the structures of 1 and 2 were identified as (23R, 24S) cimicigenol 3-O-beta-D-glucopyranosyl-(1''-->3')-beta-D-xylopyranoside and (23R, 24S) cimicigenol 3-O-beta-D-glucopyranosyl-(1''-->2')-beta-D-xylopyranoside, respectively. The in vitro immunosuppressive effects of the two new compounds 1 and 2, as well as four other known cyclolanostane saponins 3-6 on T cells were evaluated. All the agents tested effectively inhibited the proliferation of murine splenocytes induced by Concanavalin A (ConA), with IC(50) values ranging from 12.7 nM to 33.3 nM.

摘要

从升麻（毛茛科）地上部分的80%乙醇提取物中分离得到了两种新的环羊毛甾烷二糖苷，升麻苷A（1）和升麻苷B（2）。通过光谱数据和化学分析，确定1和2的结构分别为（23R，24S）升麻醇3 - O - β - D - 吡喃葡萄糖基 -（1''→3'）- β - D - 吡喃木糖苷和（23R，24S）升麻醇3 - O - β - D - 吡喃葡萄糖基 -（1''→2'）- β - D - 吡喃木糖苷。评估了两种新化合物1和2以及其他四种已知的环羊毛甾烷皂苷3 - 6对T细胞的体外免疫抑制作用。所有测试药物均有效抑制了刀豆球蛋白A（ConA）诱导的小鼠脾细胞增殖，IC（50）值范围为12.7 nM至33.3 nM。