School of Pharmaceutical Sciences, Xiamen University, 422 South Siming Road, Xiamen, Fujian 361005, PR China.
Bioorg Med Chem Lett. 2012 Mar 1;22(5):2114-8. doi: 10.1016/j.bmcl.2011.12.095. Epub 2012 Jan 16.
Among the seven natural resveratrol analogs separated and identified from Pholidota yunnanensis R(OLFE), we found phoyunbene B (PYB, trans-3,4'-dihydroxy-2',3',5-trimethoxystilbene) was more effective in inhibiting the growth of HepG2 hepatocellular carcinoma cells than resveratrol. The inhibitory effect of PYB in HepG2 cells was due to its induction of G2/M cell cycle arrest and apoptosis. Induction of G2/M phase cell cycle arrest by PYB was associated with its up-regulation of Cyclin B1, while its induction of apoptosis was accompanied with its down-regulation of Bcl-2 and up-regulation of Bax. Our in vitro invasion/migration assays also showed that PYB could inhibit the invasion of hepatocellular carcinoma cells.
从云南土沉香（Pholidota yunnanensis R(OLFE)）中分离鉴定的 7 种天然白藜芦醇类似物中，我们发现紫檀芪 B（PYB，反式-3,4'-二羟基-2',3',5-三甲氧基二苯乙烯）比白藜芦醇更能有效抑制 HepG2 肝癌细胞的生长。PYB 在 HepG2 细胞中的抑制作用是由于其诱导 G2/M 细胞周期阻滞和细胞凋亡。PYB 诱导 G2/M 期细胞周期阻滞与其上调细胞周期蛋白 B1 有关，而其诱导的细胞凋亡则伴随着 Bcl-2 的下调和 Bax 的上调。我们的体外侵袭/迁移实验也表明，PYB 可以抑制肝癌细胞的侵袭。
