Yu Xiao-Dong, Yang Jing-Lei, Zhang Wan-Lin, Liu Dong-Xu
School of Stomatology, Shandong University, No. 44-1, Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
Department of Stomatology, Qianfoshan Hospital Affiliated to Shandong University, Jinan, 250014, Shandong, China.
Tumour Biol. 2016 Mar;37(3):2871-7. doi: 10.1007/s13277-015-3793-4. Epub 2015 Sep 26.
The present study was performed to investigate the effect of resveratrol (trans-3,4',5-trihydroxystilbene) present as a natural phytoalexin in grapes, peanuts, and red wine on oral squamous cancer cell lines, SCC-VII, SCC-25, and YD-38. MTS assay and flow cytometry, respectively, were used for the analysis of inhibition of cell proliferation and apoptosis. Western blot analysis was performed to examine the effect of resveratrol on the expression of proteins associated with cell cycle regulation. The results revealed a concentration- and time-dependent inhibition of proliferation in all the three tested cell lines on treatment with resveratrol. The IC50 of resveratrol for SCC-VII, SCC-25, and YD-38 cell lines was found to be 0.5, 0.7, and 1.0 μg/ml, respectively, after 48-h treatment. Examination of the cell cycle analysis showed that resveratrol treatment induced cell cycle arrest in the G2/M phase and enhanced the expression of phospho-cdc2 (Tyr 15), cyclin A2, and cyclin B1 in the oral squamous cell carcinoma (OSCC) cells. It also caused a marked increase in the percentage of apoptotic cells as revealed by the fluorescence-activated cell sorting analysis. Thus, resveratrol exhibits inhibitory effect on the proliferation of OSCC oral cancer cells through the induction of apoptosis and G2/M phase cell cycle arrest.
本研究旨在探讨白藜芦醇（反式 - 3,4',5 - 三羟基芪）作为葡萄、花生和红酒中的天然植保素对口腔鳞状癌细胞系SCC - VII、SCC - 25和YD - 38的影响。分别采用MTS法和流式细胞术分析细胞增殖抑制和凋亡情况。进行蛋白质印迹分析以检测白藜芦醇对细胞周期调控相关蛋白表达的影响。结果显示，用白藜芦醇处理后，所有三种受试细胞系的增殖均呈现浓度和时间依赖性抑制。48小时处理后，白藜芦醇对SCC - VII、SCC - 25和YD - 38细胞系的IC50分别为0.5、0.7和1.0μg/ml。细胞周期分析表明，白藜芦醇处理诱导口腔鳞状细胞癌（OSCC）细胞在G2/M期发生细胞周期阻滞，并增强了磷酸化cdc2（Tyr 15）、细胞周期蛋白A2和细胞周期蛋白B1的表达。荧光激活细胞分选分析显示，它还导致凋亡细胞百分比显著增加。因此，白藜芦醇通过诱导凋亡和G2/M期细胞周期阻滞对OSCC口腔癌细胞的增殖表现出抑制作用。
