Department of Molecular Biology, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
Inflammation. 2012 Aug;35(4):1232-41. doi: 10.1007/s10753-012-9433-x.
Fresh rhizome of Rehmannia glutinosa Libosch. (Saeng-jihwang in Korean: SJH) has been prescribed for the treatment of diabetes-associated complications. The purpose of the present study is to investigate the underlying mechanisms of the efficacy of SJH in diabetes-related complications. Decoction was obtained after boiling SJH in water and subsequent lyophilization. The cellular toxicity of SJH was determined by MTT assay. The antioxidant activity of SJH was measured by DPPH and DCFH-DA assays. The effects of SJH on inflammatory responses elicited by AGEs were assessed by western blotting and semi-quantitative RT-PCR analyses. The water extract of SJH had a high free radical scavenging activity in vitro and decreased the level of intracellular ROS in THP-1 cells treated with AGEs. SJH suppressed the expression of pro-inflammatory genes, including TNF-α, MCP-1, IP-10, COX-2, and iNOS; the activation of NF-κB; and the expression of RAGE, a receptor for AGEs, where the expressions of which were induced by AGEs. These results suggest the possibility that SJH can be an alternative therapeutics for diabetes-associated diseases.
新鲜地黄（Saeng-jihwang 在韩语中：SJH）的根茎已被用于治疗与糖尿病相关的并发症。本研究旨在探讨 SJH 治疗糖尿病相关并发症的疗效的潜在机制。将 SJH 用水煮沸后再冻干，可获得汤剂。通过 MTT 测定法确定 SJH 的细胞毒性。通过 DPPH 和 DCFH-DA 测定法测量 SJH 的抗氧化活性。通过 Western blot 和半定量 RT-PCR 分析评估 SJH 对 AGEs 引起的炎症反应的影响。SJH 的水提取物在体外具有很高的自由基清除活性，并降低了 AGEs 处理的 THP-1 细胞内 ROS 的水平。SJH 抑制了促炎基因（包括 TNF-α、MCP-1、IP-10、COX-2 和 iNOS）的表达，抑制了 NF-κB 的激活，以及 AGEs 的受体 RAGE 的表达，而这些表达是由 AGEs 诱导的。这些结果表明，SJH 可能是治疗与糖尿病相关疾病的另一种疗法。
