MedImmune LLC, Mountain View, CA 94043, United States.
Vaccine. 2012 Mar 28;30(15):2516-22. doi: 10.1016/j.vaccine.2012.01.090. Epub 2012 Feb 11.
A miniaturized neuraminidase inhibition (NI) assay using HA-mismatched H6 reassortant viruses was performed to examine the neuraminidase (NA)-specific antibody response in ferrets immunized with live-attenuated influenza vaccine (LAIV) strains. The strains tested possessed different NAs derived from seasonal H1N1 and H3N2, 2009 pandemic H1N1, and the highly pathogenic influenza H5N1 virus. The anti-NA antibodies from the 2009 pandemic strain (A/California/7/2009) immunized ferrets cross-reacted with the NA of H5N1 but not with the NA of seasonal H1N1 viruses. The plaque size reduction assay confirmed the cross-reactivity between the NAs of A/California/7/2009 and the H5N1 virus. Sequence and structural analyses of these N1 NA proteins showed that the NA of the 2009 pandemic H1N1 strain shared at least 22 more amino acids in the head domain with the NAs of the avian H5N1 strains than with the NAs of seasonal human H1N1 viruses. Our data demonstrated LAIV-induced NA antibody responses in ferrets and cross-reactive NA antibodies induced by 2009 pandemic H1N1 and H5N1 LAIV viruses.
使用与血凝素（HA）错配的 H6 重配病毒进行了小型化神经氨酸酶抑制（NI）测定，以检查用活减毒流感疫苗（LAIV）株免疫的雪貂中的神经氨酸酶（NA）特异性抗体反应。测试的菌株具有来自季节性 H1N1 和 H3N2、2009 年大流行 H1N1 以及高致病性流感 H5N1 病毒的不同 NA。用 2009 年大流行株（A/加利福尼亚/7/2009）免疫的雪貂中的抗 NA 抗体与 H5N1 的 NA 发生交叉反应，但与季节性 H1N1 病毒的 NA 不发生交叉反应。噬菌斑减少测定法证实了 A/加利福尼亚/7/2009 与 H5N1 病毒之间的 NA 之间的交叉反应性。这些 N1 NA 蛋白的序列和结构分析表明，与季节性人 H1N1 病毒的 NA 相比，2009 年大流行 H1N1 株的 NA 在头部结构域中至少共享 22 个以上的氨基酸。我们的数据表明，LAIV 在雪貂中诱导了 NA 抗体反应，并且 2009 年大流行的 H1N1 和 H5N1 LAIV 病毒诱导了交叉反应性 NA 抗体。
