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人类卫星细胞：在人类肌纤维上的鉴定

Human satellite cells: identification on human muscle fibres.

作者信息

Boldrin Luisa, Morgan Jennifer E

机构信息

The Dubowitz Neuromuscular Centre UCL Institute of Child Health 30 Guilford Street London WC1N 1EH United Kingdom.

出版信息

PLoS Curr. 2012 Jan 19;3:RRN1294. doi: 10.1371/currents.RRN1294.

DOI:10.1371/currents.RRN1294
PMID:22333991
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3275414/
Abstract

Satellite cells, normally quiescent underneath the myofibre basal lamina, are skeletal muscle stem cells responsible for postnatal muscle growth, repair and regeneration. Since their scarcity and small size have limited study on transverse muscle sections, techniques to isolate individual myofibres, bearing their attendant satellite cells, were developed. Studies on mouse myofibres have generated much information on satellite cells, but the limited availability and small size of human muscle biopsies have hampered equivalent studies of satellite cells on human myofibres. Here, we identified satellite cells on fragments of human and mouse myofibres, using a method applicable to small muscle biopsies.

摘要

卫星细胞通常静息于肌纤维基膜下方，是负责出生后肌肉生长、修复和再生的骨骼肌干细胞。由于其数量稀少且体积微小，限制了对横向肌切片的研究，因此人们开发了分离带有卫星细胞的单个肌纤维的技术。对小鼠肌纤维的研究已产生了许多关于卫星细胞的信息，但人类肌肉活检样本的有限可用性和小尺寸阻碍了对人类肌纤维上卫星细胞的等效研究。在此，我们使用一种适用于小肌肉活检样本的方法，鉴定了人类和小鼠肌纤维片段上的卫星细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/4dd58efe4f7f/figure-3-new.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/9df70d4ff31e/figure-1-new.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/8372b18b8221/figure-2-new.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/4dd58efe4f7f/figure-3-new.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/9df70d4ff31e/figure-1-new.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/8372b18b8221/figure-2-new.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2e/3275414/4dd58efe4f7f/figure-3-new.jpg

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本文引用的文献

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