Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C.-CIATEJ, Guadalajara, Jalisco, Mexico.
Nutr Neurosci. 2012 Mar;15(2):62-9. doi: 10.1179/1476830511Y.0000000034. Epub 2012 Feb 2.
One of the main concerns regarding organophosphate pesticides (OP) is their possible toxic effects. Doses that do not produce acute toxicity are capable of altering the structure and biochemistry of different tissues and organs by production of reactive oxygen species (ROS). Curcumin (CUR) is the main substance in Curcuma longa (Zingiberacea) rhizome that has strong antioxidant activity. However, the neuroprotective properties of curcumin against oxidative stress induced by prolonged exposure to parathion (PAR) is not clear.
The present work evaluated the protective effect of curcumin against the oxidative damage induced in the rat hippocampus by the OP PAR.
Forty female Wistar rats were distributed in four groups as follows: exposed to PAR by inhalation (PAR group); pre-treated with CUR and then exposed to PAR by inhalation, (CUR + PAR group); exposed to environmental air and treated with CUR in the food (CUR group); and exposed to environmental air (the control group). At the end of the handling process, the concentration of erythrocyte cholinesterase was monitored, as indicator of PAR intoxication and lipoperoxidation, immunohistochemistry for astrocytes, and activated microglia and apoptosis was determined in the hippocampus.
In the present study, we show that the administration of CUR (200 mg/kg body weight) significantly diminished the oxidative damage in the hippocampus of rats exposed to the OP PAR.
These data suggest that CUR may be an alternative to prevent neurodegenerative damage after pesticide exposure.
人们主要关注有机磷农药（OP）的可能毒性作用。不会产生急性毒性的剂量能够通过产生活性氧（ROS）来改变不同组织和器官的结构和生物化学性质。姜黄素（CUR）是姜黄（Zingiberacea）根茎中的主要物质，具有很强的抗氧化活性。然而，姜黄素对久效磷（PAR）引起的氧化应激的神经保护特性尚不清楚。
本工作评估了姜黄素对大鼠海马中由 OP PAR 诱导的氧化损伤的保护作用。
将 40 只雌性 Wistar 大鼠分为四组：吸入 PAR（PAR 组）；先用 CUR 预处理，然后吸入 PAR（CUR + PAR 组）；暴露于环境空气中并用食物中的 CUR 处理（CUR 组）；暴露于环境空气中（对照组）。在处理过程结束时，监测红细胞乙酰胆碱酯酶的浓度，作为 PAR 中毒和脂质过氧化的指标，用免疫组织化学法测定海马中的星形胶质细胞、活化的小胶质细胞和细胞凋亡。
本研究表明，CUR（200mg/kg 体重）的给药可显著减轻暴露于 OP PAR 的大鼠海马中的氧化损伤。
这些数据表明，CUR 可能是预防农药暴露后神经退行性损伤的一种替代方法。
