Laboratorio de Neurofisiología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, Mexico.
Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Oxid Med Cell Longev. 2018 Dec 30;2018:9620684. doi: 10.1155/2018/9620684. eCollection 2018.
Ozone is a harmful tropospheric pollutant, causing the formation of reactive oxygen and nitrogen species that lead to oxidative damage in living beings. NF-B can be activated in response to oxidative damage, inducing an inflammatory response. Nowadays, there are no reliable results that consolidate the use of antioxidants to protect from damage caused by ozone, particularly in highly polluted cities. Curcumin has a strong antioxidant activity and is a potent inhibitor of NF-B activation with no side effects. The aim of this study is to evaluate the effect of curcumin in preventive and therapeutic approaches against oxidative damage, NF-B activation, and the rise in serum levels of IL-1 and TNF- induced by acute and chronic exposure to ozone in rat hippocampus. One hundred male Wistar rats were distributed into five groups; the intact control, curcumin-fed control, the ozone-exposed group, and the preventive and therapeutic groups. These last two groups were exposed to ozone and received food supplemented with curcumin. Lipid peroxidation was determined by spectrophotometry, and protein oxidation was evaluated by immunodetection of carbonylated proteins and densitometry analysis. Activation of NF-B was assessed by electrophoretic mobility shift assay (EMSA), and inflammatory cytokines (IL-1 and TNF-) were determined by ELISA. Curcumin decreased NF-B activation and serum levels of inflammatory cytokines as well as protein and lipid oxidation, in both therapeutic and preventive approaches. Curcumin has proven to be a phytodrug against the damage caused by the environmental exposure to ozone.
臭氧是一种有害的对流层污染物，会形成活性氧和氮物种，导致生物体内的氧化损伤。NF-B 可以被氧化损伤激活，从而诱导炎症反应。目前，没有可靠的结果证实抗氧化剂的使用可以保护生物体免受臭氧造成的损伤，尤其是在污染严重的城市。姜黄素具有很强的抗氧化活性，是 NF-B 激活的有效抑制剂，且无副作用。本研究旨在评估姜黄素在预防和治疗急性和慢性暴露于臭氧引起的大鼠海马氧化损伤、NF-B 激活以及血清中白细胞介素-1（IL-1）和肿瘤坏死因子-α（TNF-α）水平升高方面的作用。将 100 只雄性 Wistar 大鼠随机分为五组：完整对照组、姜黄素喂养对照组、臭氧暴露组、预防组和治疗组。后两组暴露于臭氧，并接受补充姜黄素的食物。通过分光光度法测定脂质过氧化，通过免疫检测羰基化蛋白和密度分析评估蛋白质氧化。通过电泳迁移率变动分析（EMSA）评估 NF-B 的激活，通过 ELISA 测定炎症细胞因子（IL-1 和 TNF-α）。姜黄素在治疗和预防两种方法中均降低了 NF-B 激活和血清中炎症细胞因子以及蛋白质和脂质氧化的水平。姜黄素已被证明是一种植物药物，可以对抗环境暴露于臭氧造成的损伤。
