香菇多糖和提取物：结构特征和抗病毒活性。
Polysaccharide and extracts from Lentinula edodes: structural features and antiviral activity.
机构信息
Departamento de Microbiologia, Universidade Estadual de Londrina, Rod, Celso Garcia Cid,, km 380, CEP: 86051-990, Londrina, Paraná, Brazil.
出版信息
Virol J. 2012 Feb 15;9:37. doi: 10.1186/1743-422X-9-37.
BACKGROUND
Lentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, moreover, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. The aim of this study was to determine the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts and polysaccharide (LeP) from Lentinula edodes in the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1).
METHODS
The time-of-addition assay was performed at the times -2, -1, 0, 1 and 2 h of the infection. The virucidal activity and the inhibition of viral adsorption were also evaluated. Plaque assay was used to monitor antiviral activity throughout.
RESULTS
The AqE and LeP were more effective when added at 0 h of infection, however, EtOHE was more effective at the times 1 h and 2 h of the infection. AqE, EtOHE and LeP showed low virucidal activity, and the inhibition of viral adsorption was not significant.
CONCLUSIONS
The results allowed us to conclude that AqE, EtOHE and LeP act on the initial processes of the replication of both strains of virus.
背景
香菇，又称“花菇”，不仅可食用，还被广泛应用于民间医学。此外，从其菌丝体和子实体中分离得到的化合物已显示出多种治疗特性。本研究旨在确定香菇的水（AqE）和乙醇（EtOHE）提取物及多糖（LeP）对 1 型脊髓灰质炎病毒（PV-1）和 1 型牛疱疹病毒（BoHV-1）复制的抗病毒活性。
方法
在感染前 -2、-1、0、1 和 2 小时进行时间添加测定。还评估了病毒灭活活性和病毒吸附抑制作用。通过噬菌斑测定法监测整个抗病毒活性。
结果
AqE 和 LeP 在感染 0 小时时效果更好，而 EtOHE 在感染 1 小时和 2 小时时效果更好。AqE、EtOHE 和 LeP 表现出较低的病毒灭活活性，对病毒吸附的抑制作用不明显。
结论
研究结果表明，AqE、EtOHE 和 LeP 作用于两种病毒株复制的初始阶段。
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