Department of Ophthalmology, Medical University in Lublin, Lublin, Poland.
Pharm Biol. 2012 Feb;50(2):147-54. doi: 10.3109/13880209.2011.579980.
Ocular diseases are currently an important problem in modern societies. Patients suffer from various ophthalmologic ailments namely, conjunctivitis, dry eye, dacryocystitis or degenerative diseases. Therefore, there is a need to introduce new treatment methods, including medicinal plants usage. Aloe vera [Aloe barbadensis Miller (Liliaceae)] possesses wound-healing properties and shows immunomodulatory, anti-inflammatory or antioxidant activities.
NR uptake, MTT, DPPH• reduction, Griess reaction, ELISA and rhodamine-phalloidin staining were used to test toxicity, antiproliferative activity, reactive oxygen species (ROS) reduction, nitric oxide (NO) and cytokine level, and distribution of F-actin in cells, respectively.
The present study analyzes the effect of Aloe vera extracts obtained with different solvents on in vitro culture of human 10.014 pRSV-T corneal cells.
We found no toxicity of ethanol, ethyl acetate and heptane extracts of Aloe vera on human corneal cells. No ROS reducing activity by heptane extract and trace action by ethanol (only at high concentration 125 µg/ml) extract of Aloe vera was observed. Only ethyl acetate extract expressed distinct free radical scavenging effect. Plant extracts decreased NO production by human corneal cells as compared to untreated controls. The cytokine (IL-1β, IL-6, TNF-α and IL-10) production decreased after the addition of Aloe vera extracts to the culture media.
Aloe vera contains multiple pharmacologically active substances which are capable of modulating cellular phenotypes and functions. Aloe vera ethanol and ethyl acetate extracts may be used in eye drops to treat inflammations and other ailments of external parts of the eye such as the cornea.
眼部疾病是现代社会的一个重要问题。患者患有各种眼科疾病，如结膜炎、干眼症、泪囊炎或退行性疾病。因此，需要引入新的治疗方法，包括使用药用植物。芦荟[Aloe barbadensis Miller（百合科）]具有愈合伤口的特性，并具有免疫调节、抗炎或抗氧化作用。
使用 NR 摄取、MTT、DPPH•还原、Griess 反应、ELISA 和罗丹明鬼笔环肽染色分别测试毒性、抗增殖活性、活性氧（ROS）减少、一氧化氮（NO）和细胞因子水平以及 F-肌动蛋白在细胞中的分布。
本研究分析了用不同溶剂获得的芦荟提取物对体外培养的人 10.014 pRSV-T 角膜细胞的影响。
我们发现芦荟的乙醇、乙酸乙酯和正庚烷提取物对人角膜细胞没有毒性。正庚烷提取物没有 ROS 还原活性，而只有乙醇提取物（仅在高浓度 125µg/ml 时）有轻微作用。只有乙酸乙酯提取物表现出明显的自由基清除作用。与未经处理的对照相比，植物提取物减少了人角膜细胞的 NO 产生。加入芦荟提取物后，细胞因子（IL-1β、IL-6、TNF-α 和 IL-10）的产生减少。
芦荟含有多种具有调节细胞表型和功能的药理活性物质。芦荟的乙醇和乙酸乙酯提取物可用于滴眼剂，以治疗眼部外部炎症和其他疾病，如角膜。
