School of Bioscience and Engineering, Jadavpur University, Kolkata, India.
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
Nat Prod Res. 2020 Aug;34(16):2363-2366. doi: 10.1080/14786419.2018.1534848. Epub 2019 Jan 2.
leaf contains some bioactive compounds that have a strong binding affinity toward estrogen receptor as compared to standard drug tamoxifen. In this study, we have found that the IC of leaf extract against breast cancer cell line (MCF-7) is 23 µg/mL which is much lower than the IC (332 µg/mL) of against non-cancerous cell line (NIH-3T3). We have also calculated the total concentration of phenolic acid (385.662 µg/mL), flavonoids (160.402 µg/mL) and alkaloids (276.754 µg/mL) in leaf extract. The free radical scavenging activity of leaf extract is 67% to 89% (at 50 to 300 µg/ml). Our virtual molecular docking study suggests that bioactive compounds like Aloe-emodin (-8.8 Kcal/mol), 7-hydroxy-2,5 dimethylchromone (-7.5 Kcal/mol), Beta-sitosterol (-7.3 Kcal/mol) etc. have a greater binding affinity toward estrogen alpha receptor as compared to standard drug Tamoxifen (-6.4 Kcal/mol). [Formula: see text].
叶含有一些生物活性化合物，与标准药物他莫昔芬相比，对雌激素受体具有很强的结合亲和力。在这项研究中，我们发现叶提取物对乳腺癌细胞系（MCF-7）的 IC 为 23μg/mL，远低于非癌细胞系（NIH-3T3）的 IC（332μg/mL）。我们还计算了叶提取物中总酚酸（385.662μg/mL）、类黄酮（160.402μg/mL）和生物碱（276.754μg/mL）的浓度。叶提取物的自由基清除活性为 67%至 89%（在 50 至 300μg/ml 之间）。我们的虚拟分子对接研究表明，生物活性化合物，如芦荟大黄素（-8.8kcal/mol）、7-羟基-2,5-二甲基色酮（-7.5kcal/mol）、β-谷甾醇（-7.3kcal/mol）等，与标准药物他莫昔芬（-6.4kcal/mol）相比，对雌激素α受体具有更强的结合亲和力。[公式：见正文]。
