Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Tunisia.
Lipids Health Dis. 2011 Feb 11;10:30. doi: 10.1186/1476-511X-10-30.
In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS) extracts by using various solvents (hexane, chloroform-ethanol (1/1), ethyl acetate, butanol and water). We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA).
The water extract exhibits the highest inhibitory effect with an IC50 = 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB) even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1) and ethyl acetate (IC50 = 0.274 and 0.326 mg/ml, respectively). Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g). This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively), mostly responsible for the antioxidant activity.
A significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds.
在本工作中，我们使用各种溶剂（正己烷、氯仿-乙醇（1/1）、乙酸乙酯、正丁醇和水）测定了库拉索芦荟叶皮（AVLS）提取物中的总酚含量。我们还通过测量这些提取物对人源促炎磷脂酶 A2（IIA 组）的抑制能力来评估它们的抗氧化和抗 PLA2 特性。
水提取物表现出最高的抑制作用，IC50=0.22mg/ml，有趣的是，即使在 5mg/ml 的浓度下，对消化性磷脂酶 A2（IB 组）也没有观察到任何作用。还分析了抗氧化活性，当使用氯仿-乙醇（1/1）和乙酸乙酯时，观察到最活跃的提取物（IC50=0.274 和 0.326mg/ml，分别）。总酚含量分析表明，具有最佳抗 PLA2 作用的水提取物中酚类分子含量低（2mgGAE/g）。这一数值必须与氯仿-乙醇和乙酸乙酯提取物（分别为 40 和 23.8mgGAE/g）进行比较，这两种提取物主要负责抗氧化活性。
总酚含量与抗氧化能力之间存在显著相关性，但与抗 PLA2 活性无关。植物化学筛选结果表明，抗 PLA2 分子可能是儿茶素单宁化合物。
