Rosellen Jens, Fritzenwanker Moritz, Schuppe Hans-Christian, Schagdarsurengin Undraga, Wagenlehner Florian, Pilatz Adrian
Department of Urology, Pediatric Urology and Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany.
Institute for Medical Microbiology, Justus Liebig University Giessen, 35390 Giessen, Germany.
Diagnostics (Basel). 2025 Apr 15;15(8):1003. doi: 10.3390/diagnostics15081003.
: 16S rRNA analysis has been used in various diseases to identify pathogenic bacteria. In particular, pathogens that are difficult to cultivate or previously unknown can be detected with great certainty. In chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), a distinction between bacterial and non-bacterial genesis is essential with regard to categorization and therapy. The objective of this study is to investigate the value of 16S rRNA gene sequence analysis in the routine management of patients with CP/CPPS especially after failure to detect a pathogen in conventional culture and polymerase chain reaction for sexually transmitted diseases (STI-PCR). : In total, 228 patients with CP/CPPS were prospectively enrolled and received a comprehensive andrological work-up. Microbial analysis consisted of standard bacterial cultures and the detection of sexually transmitted pathogens by PCR using urine specimens from a 2-glass test and semen analysis. 16S rRNA gene sequence analysis was performed in patients with urine and semen of patients without bacterial pathogens in microbiological culture and STI-PCR. : In 184 of 199 (92%) patients with negative ejaculate culture and negative STI-PCR, no pathogen could be detected by 16S rRNA analysis and in the case of a positive result, the analysis only showed non-pathogenic bacteria of the normal flora. There was no statistical association between the 16S rRNA analysis and the inflammatory markers or the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) scores. : At least in our study cohort, the 16S rRNA analysis provided no additional benefit following microbiological culture and STI-PCR in the categorization of patients with CP/CPPS.
16S rRNA分析已被用于多种疾病中以鉴定病原菌。特别是，难以培养或先前未知的病原体能够被高度准确地检测出来。在慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）中，区分细菌性和非细菌性病因对于分类和治疗至关重要。本研究的目的是探讨16S rRNA基因序列分析在CP/CPPS患者常规管理中的价值，尤其是在常规培养和性传播疾病聚合酶链反应（STI-PCR）未能检测到病原体之后。总共前瞻性纳入了228例CP/CPPS患者，并对其进行了全面的男科检查。微生物分析包括标准细菌培养以及通过使用两杯试验的尿液标本和精液分析进行PCR检测性传播病原体。对微生物培养和STI-PCR中无细菌病原体的患者的尿液和精液进行16S rRNA基因序列分析。在199例射精培养阴性和STI-PCR阴性的患者中，有184例（92%）通过16S rRNA分析未检测到病原体，而在结果为阳性的情况下，分析仅显示正常菌群中的非病原菌。16S rRNA分析与炎症标志物或美国国立卫生研究院慢性前列腺炎症状指数（NIH-CPSI）评分之间无统计学关联。至少在我们的研究队列中，在对CP/CPPS患者进行分类时，16S rRNA分析在微生物培养和STI-PCR之后没有提供额外的益处。
