Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
World J Gastroenterol. 2012 Feb 7;18(5):479-88. doi: 10.3748/wjg.v18.i5.479.
To assess the safety of Bifidobacterium longum (B. longum) JDM301 based on complete genome sequences.
The complete genome sequences of JDM301 were determined using the GS 20 system. Putative virulence factors, putative antibiotic resistance genes and genes encoding enzymes responsible for harmful metabolites were identified by blast with virulence factors database, antibiotic resistance genes database and genes associated with harmful metabolites in previous reports. Minimum inhibitory concentration of 16 common antimicrobial agents was evaluated by E-test.
JDM301 was shown to contain 36 genes associated with antibiotic resistance, 5 enzymes related to harmful metabolites and 162 nonspecific virulence factors mainly associated with transcriptional regulation, adhesion, sugar and amino acid transport. B. longum JDM301 was intrinsically resistant to ciprofloxacin, amikacin, gentamicin and streptomycin and susceptible to vancomycin, amoxicillin, cephalothin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, imipenem and trimethoprim-sulphamethoxazol. JDM301 was moderately resistant to bacitracin, while an earlier study showed that bifidobacteria were susceptible to this antibiotic. A tetracycline resistance gene with the risk of transfer was found in JDM301, which needs to be experimentally validated.
The safety assessment of JDM301 using information derived from complete bacterial genome will contribute to a wider and deeper insight into the safety of probiotic bacteria.
基于全基因组序列评估长双歧杆菌（B. longum）JDM301 的安全性。
使用 GS 20 系统测定 JDM301 的全基因组序列。通过与先前报道的毒力因子数据库、抗生素耐药基因数据库和与有害代谢物相关的基因进行blast 分析，鉴定潜在的毒力因子、潜在的抗生素耐药基因和负责有害代谢物的编码酶。采用 E 试验评估 16 种常见抗菌药物的最小抑菌浓度。
JDM301 含有 36 个与抗生素耐药相关的基因、5 个与有害代谢物相关的酶和 162 个非特异性毒力因子，主要与转录调控、黏附、糖和氨基酸转运有关。长双歧杆菌 JDM301 对环丙沙星、阿米卡星、庆大霉素和链霉素固有耐药，对万古霉素、阿莫西林、头孢噻吩、氯霉素、红霉素、氨苄西林、头孢噻肟、利福平、亚胺培南和复方磺胺甲噁唑敏感。JDM301 对杆菌肽中度耐药，而先前的研究表明双歧杆菌对该抗生素敏感。在 JDM301 中发现了一个具有转移风险的四环素耐药基因，需要进行实验验证。
使用源自全细菌基因组的信息对 JDM301 进行安全性评估，有助于更广泛和深入地了解益生菌的安全性。
