University of Nis, Faculty of Technology, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia; E-Mails:
Sensors (Basel). 2009;9(7):5702-14. doi: 10.3390/s90705702. Epub 2009 Jul 16.
The antioxidant activity of water, ethanol and methanol Hieracium pilosella L. extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH(•) free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH(•) radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59-244.16 mg GAE/g of dry extract) and total flavonoids (79.13-82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity.
报道了委陵菜水、乙醇和甲醇提取物的抗氧化活性。采用分光光度法，通过测量它们清除稳定的 DPPH（•）自由基和 Fenton 反应中 DMPO 捕获的反应性羟基自由基的能力，用 ESR 光谱法测试了抗氧化活性。根据福林-考尔法测定总酚含量，根据比色法测定总黄酮含量。使用 HPLC 方法鉴定了一些酚类化合物（绿原酸、芹菜素-7-O-葡萄糖苷和伞形酮）。所研究的提取物的抗氧化活性因所用溶剂的不同而略有差异。浓度为 0.30 mg/mL 的水、乙醇和甲醇提取物在清除羟基自由基（分别为 56.35%、58.73%和 54.35%）方面的效果不如 DPPH（•）自由基清除活性（所有提取物均接近 95%）。高含量的总酚类化合物（239.59-244.16 mg GAE/g 干提取物）和总黄酮类化合物（79.13-82.18 mg RE/g 干提取物）表明这些化合物有助于抗氧化活性。
