Abarca-Vargas Rodolfo, Zamilpa Alejandro, Petricevich Vera L
Facultad de Medicina de la, Universidad Autónoma del Estado de Morelos (UAEM), Calle: Leñeros, esquina Iztaccíhuatl s/n. Col. Volcanes, Cuernavaca, Mor. C.P. 62350, Mexico.
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Xochitepec CP 62790, Morelos, Mexico.
Antioxidants (Basel). 2019 Aug 1;8(8):264. doi: 10.3390/antiox8080264.
In this study the effect of the ethanol concentration of extracts on the flavonoids content, and its antioxidant and cytoprotective activities in vitro were determined and compared. For the elucidation of the chemical constituents, the high-performance liquid chromatography method (HPLC) was used, and verification of the antioxidant activity was carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. The cytoprotective effects of extracts were determined by exposure to hydrogen peroxide. The HPLC analysis showed the presence of rutin, quercetin-3-glucoside and quercetin rhamnoside. Among the extracts investigated the best recuperation of the rutin content was observed in extracts with 80% ethanol (83 ± 5 mg/mL). The amounts of rutin present in all extracts contribute to the antioxidant capacity and the IC was 427.49 (0%), 275.41 (50%), 271.61 (80%), and 272.14 (100%) µg/mL. The lowest percentage of viability was found in the cultures exposed to 100% ethanol (92%). In cultures exposed to hydrogen peroxide the percentages of protection were 25%, 33%, 78%, and 65% for cultures treated for 72 h at 0%, 50%, 80%, and 100% ethanol, respectively. The ethanolic extract of was confirmed to have high rutin content with potent antioxidant activity, low cytotoxic and strong cytoprotective effects.
在本研究中，测定并比较了提取物的乙醇浓度对黄酮类化合物含量及其体外抗氧化和细胞保护活性的影响。为阐明化学成分，采用了高效液相色谱法（HPLC），并使用2,2-二苯基-1-苦基肼基（DPPH）自由基法对抗氧化活性进行了验证。通过暴露于过氧化氢来测定提取物的细胞保护作用。HPLC分析表明存在芦丁、槲皮素-3-葡萄糖苷和槲皮素鼠李糖苷。在所研究的提取物中，在80%乙醇提取物中观察到芦丁含量的最佳恢复情况（83±5mg/mL）。所有提取物中芦丁的含量有助于抗氧化能力，其IC50分别为427.49（0%）、275.41（50%）、271.61（80%）和272.14（100%）μg/mL。在暴露于100%乙醇的培养物中发现最低的活力百分比（92%）。在暴露于过氧化氢的培养物中，0%、50%、80%和100%乙醇处理72小时的培养物的保护百分比分别为25%、33%、78%和65%。已证实[提取物名称未给出]的乙醇提取物具有高芦丁含量、强大的抗氧化活性、低细胞毒性和强大的细胞保护作用。
