伊朗西南部胡齐斯坦省利什曼原虫物种的微外显子基因分型
Mini-exon genotyping of leishmania species in khuzestan province, southwest iran.
作者信息
Saki J, Meamar Ar, Oormazdi H, Akhlaghi L, Maraghi S, Mohebali M, Khademvatan S, Razmjou E
机构信息
Department of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Parasitol. 2010 Mar;5(1):25-34.
BACKGROUND
Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoonotic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest of Iran.
METHODS
From 2007 to 2008 in this cross-sectional study, cutaneous samples were collected from patients referred to Health Centers and Hospitals of the Khuzestan Province for cutaneous leishmaniasis diagnosis and cultured in Novy-MacNeal-Nicolle (NNN) and RPMI 1640. The propagated promastigotes were harvested and Leishmania species of cutaneous leishmaniasis were identified by RFLP and DNA sequencing of the PCR generated fragments.
RESULTS
L. major and L. tropica were the causative agents of cutaneous leishmaniasis by predominantly of L. major species. The alignment of the mini-exon sequencing isolates with reported sequencing of L. major and L. tropica revealed 92%-99% identity.
CONCLUSION
Our study showed that mini-exon PCR-RFLP was useful method to identify the causative species of cutaneous leishmaniasis.
背景
利什曼病是由利什曼原虫属引起的原生动物疾病。人源性和动物源性皮肤利什曼病在伊朗为地方病。本研究的目的是通过微小外显子基因鉴定伊朗西南部胡齐斯坦省五个地区皮肤利什曼病的病原体。
方法
在2007年至2008年的这项横断面研究中，从转诊至胡齐斯坦省卫生中心和医院进行皮肤利什曼病诊断的患者中采集皮肤样本，并在诺维 - 麦克尼尔 - 尼科尔（NNN）培养基和RPMI 1640培养基中培养。收获增殖的前鞭毛体，并通过PCR扩增片段的RFLP和DNA测序鉴定皮肤利什曼病的利什曼原虫种类。
结果
硕大利什曼原虫和热带利什曼原虫是皮肤利什曼病的病原体，主要为硕大利什曼原虫。微小外显子测序分离株与已报道的硕大利什曼原虫和热带利什曼原虫测序结果的比对显示同一性为92% - 99%。
结论
我们的研究表明，微小外显子PCR - RFLP是鉴定皮肤利什曼病病原体种类的有用方法。
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