Hezari Farideh, Niyyati Maryam, Seyyed Tabaei Seyyed Javad, Mohebali Mehdi, Moin Vaziri Vahideh, Behniafar Hamed, Azargashb Eznolah, Taghipour Niloofar
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2016 Oct;45(10):1348-1354.
Leishmaniasis is a zoonotic disease caused by species of protozoa of the genus . In recent years, incidence of cutaneous leishmaniasis has increasing trend in Golestan Province, North of Iran. The aim of the present study was to identify the frequency of cutaneous leishmaniasis using PCR-RFLP in patients referred to Kalaleh Health Center, during 2013-14.
This descriptive cross-sectional study was conducted on 70 individuals with suspected cutaneous leishmaniasis that referred to health center of Kalaleh County, Golestan Province, Northern Iran, from Sep 2013 to Nov 2014. Samples of cutaneous lesions were examined microscopically. DNA was extracted from all of the positive smears and PCR was done on ITS-1 gene. RFLP was performed using HaeIII enzyme for species identification.
Totally, 38 out of the 70 (54.3%) suspected individuals including 22 males (57.9%) were found positive by microscopic examination. All of microscopically positive samples were confirmed to be positive for DNA (approximately 340 bp bands were detected). RFLP revealed 140 bp and 200 bp bands (approximate size), indicative of .
The detected species of studied region was . Cutaneous leishmaniasis has high prevalence in Kalaleh County, thus more studies on leishmaniasis in the animal reservoirs, comparison of homology of animal and human isolates and a survey regarding natural infection of vectors in this region is highly recommended.
利什曼病是由利什曼原虫属的原生动物引起的一种人畜共患病。近年来，伊朗北部戈勒斯坦省皮肤利什曼病的发病率呈上升趋势。本研究的目的是在2013 - 2014年期间，使用聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）技术，对转诊至卡拉莱健康中心的患者中皮肤利什曼病的感染率进行鉴定。
本描述性横断面研究于2013年9月至2014年11月对70例疑似皮肤利什曼病患者进行，这些患者转诊至伊朗北部戈勒斯坦省卡拉莱县健康中心。对皮肤病变样本进行显微镜检查。从所有阳性涂片提取DNA，并对ITS - 1基因进行PCR扩增。使用HaeIII酶进行RFLP分析以鉴定物种。
在70例疑似患者中，共有38例（54.3%）通过显微镜检查呈阳性，其中包括22名男性（57.9%）。所有显微镜检查阳性的样本经确认均为利什曼原虫DNA阳性（检测到约340 bp条带）。RFLP分析显示出140 bp和200 bp条带（近似大小），表明为某种利什曼原虫。
研究区域检测到的利什曼原虫种类为某种利什曼原虫。皮肤利什曼病在卡拉莱县的患病率很高，因此强烈建议对该地区动物宿主中的利什曼病进行更多研究，比较动物和人类分离株的同源性，并对媒介的自然感染情况进行调查。
