Eslami Gilda, Fattahi Bafghi Ali, Lotfi Mohammad Hassan, Mirzaei Farzaneh, Ahmadi Somayeh, Tajfirouzeh Ali Akbar, Jafarizadeh Hamid, Pormazar Sayyed Alireza, Vakili Mahmoud
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran.
Deputy of Health, Health System Research Unit, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Public Health. 2020 May;49(5):975-980.
Cutaneous Leishmaniasis (CL) is a major health problem in many parts of Iran. Many methods have been introduced for detection and identification of Cutaneous Leishmaniasis. The purpose of this study was isolation and molecular identification of spp. agents in patients with CL from endemic region of central Iran. In this study, one of the main loci of central Iran named Yazd will be assessed CL identification using PCR-RFLP.
For this cross-sectional study, sampling was done from 372 suspicious patients with CL who referred to Health Centers of Yazd Province from 2016 to 2017. After collection samples of patients, DNA extraction was done from samples on slides. Genus detection was done using specific primers by PCR. RFLP analysis was done for species identification.
Out of 372 samples, 159 samples were positive using PCR based method. Out of 159 samples, 87 (54.7%) and 72 (45.3%) were identified using RFLP analysis. The number of lesions in each patient was different but 119 (74.8%) patients showed the number of 1-3 lesions, and more lesions (more than 10 lesions) was showed in 4 (2.5%) person.
The CL found in Yazd province resulted from and as the agents of rural and urban types, respectively. The prevalence of L. major and was almost the same This indicated that control programs could be designed for treatment and vector and reservoir control.
皮肤利什曼病（CL）是伊朗许多地区的一个主要健康问题。已经介绍了许多用于检测和鉴定皮肤利什曼病的方法。本研究的目的是从伊朗中部流行地区的CL患者中分离和分子鉴定利什曼原虫属物种。在本研究中，将使用PCR-RFLP评估伊朗中部一个名为亚兹德的主要地区的CL鉴定情况。
对于这项横断面研究，从2016年至2017年转诊至亚兹德省卫生中心的372例疑似CL患者中进行采样。收集患者样本后，从载玻片上的样本中提取DNA。通过PCR使用特异性引物进行属检测。进行RFLP分析以进行物种鉴定。
在372个样本中，基于PCR的方法有159个样本呈阳性。在159个样本中，通过RFLP分析鉴定出87个（54.7%）和72个（45.3%）。每位患者的病变数量不同，但119名（74.8%）患者有1-3个病变，4名（2.5%）患者有更多病变（超过10个病变）。
在亚兹德省发现的CL分别由硕大利什曼原虫和热带利什曼原虫引起，它们分别是农村型和城市型的病原体。硕大利什曼原虫和热带利什曼原虫的患病率几乎相同。这表明可以设计控制方案来进行治疗以及控制媒介和宿主。
