单独使用维生素D3以及与干扰素-γ混合使用对刚地弓形虫（RH株）速殖子在BALB/C小鼠感染巨噬细胞中增殖及一氧化氮（NO）产生的影响
The Effect of Vitamin D3 Alone and Mixed With IFN-γ on Tachyzoites of Toxoplasma gondii (RH Strain) Proliferation and Nitric Oxide (NO) Production in Infected Macrophages of BALB/C Mice.
作者信息
Ghaffarifar F, Abdolah Pour M, Sharifi Z, Dalimi Asl A, Al-Kawaz E
机构信息
Department of Parasitology, Tarbiat Modarres University, Tehran, Iran.
出版信息
Iran J Parasitol. 2010 Sep;5(3):48-56.
INTRODUCTION
Toxoplasma gondii is an obligatory interacelullar parasite that infects nucleated cells in its intermediate hosts. The aim of the present study was to determine the effect of vitamin D3 on the multiplication of T. gondii in peritoneal macrophage of Balb/c mice and nitric oxide production by macrophages.
METHODS
According to usage of vitamin D3 (one dose or seven doses) and INFγ in vitro and in vivo, this study was divided into four experiments. In all experiments, the macrophages were collected from peritoneum and cultured in RPMI-1640. Then the supernatants were collected after 24 h and their nitric oxide was measure. After 96 h, the macrophages were collected and stained and the number of tachyzoites was measured.
RESULTS
The first experiment (the mice were infected with tachyzoites and after 2 h, got one dose vitamin D3 intraperitonealy) showed the best results. The mean of tachyzoites per macrophages was 2.37, and mean±SD of nitric oxide was 187.8±9.
DISCUSSION
High-level production of nitric oxide may be related to the only one injection of vitamin D3. The injection in long time might suppress the immune system.
引言
刚地弓形虫是一种专性细胞内寄生虫，可感染其中间宿主的有核细胞。本研究的目的是确定维生素D3对刚地弓形虫在Balb/c小鼠腹腔巨噬细胞中增殖的影响以及巨噬细胞产生一氧化氮的情况。
方法
根据维生素D3（一剂或七剂）和干扰素γ在体外和体内的使用情况，本研究分为四个实验。在所有实验中，从腹膜收集巨噬细胞并在RPMI-1640中培养。然后在24小时后收集上清液并测量其一氧化氮含量。96小时后，收集巨噬细胞进行染色并测量速殖子数量。
结果
第一个实验（小鼠感染速殖子后2小时腹腔注射一剂维生素D3）显示出最佳结果。每个巨噬细胞中速殖子的平均数为2.37，一氧化氮的平均值±标准差为187.8±9。
讨论
高水平的一氧化氮产生可能与仅注射一剂维生素D3有关。长时间注射可能会抑制免疫系统。
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