Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Mol Biol Rep. 2012 Jun;39(6):6967-74. doi: 10.1007/s11033-012-1524-0. Epub 2012 Feb 16.
A MADS box gene AGL20/SOC1 is a main integrator in Arabidopsis flowering pathway whose structure and function are highly conserved in many plant species. A soybean MADS box gene GmGAL1 (Glycine max AGAMOUS Like 1) as a homolog of AGL20/SOC1, was cloned from soybean cultivar Kennong18 and its function was investigated in transgenic Arabidopsis lines. Sequence comparisons showed that the closest homolog gene to GmGAL1 is AGL20/SOC1 in Arabidopsis and VuSOC1 in Vigna unguiculata. We investigated the expression level of GmGAL1 using quantitative real-time PCR, and the result showed that GmGAL1 was regulated by a circadian mechanism and its expression oscillated at a cycle of 24 h. The expression level of GmGAL1 was fluctuated in diverse tissues/organs and developmental stages. Considering its expression can be detected in different tissues throughout the life cycle and its protein localized in cytoplasm in Arabidopsis protoplasm, we proposed that GmGAL1 may be a multifunctional gene in the context of the soybean development. Ectopic expression of GmGAL1 in Arabidopsis enhanced flowering under long-day condition and partially rescued soc1 late flowering type.
一个 MADS 框基因 AGL20/SOC1 是拟南芥开花途径中的主要整合因子，其结构和功能在许多植物物种中高度保守。大豆 MADS 框基因 GmGAL1（大豆 AGAMOUS 样 1）是 AGL20/SOC1 的同源物，从大豆品种 Kennong18 中克隆出来，并在转基因拟南芥品系中研究了其功能。序列比较表明，与 GmGAL1 最接近的同源基因是拟南芥中的 AGL20/SOC1 和豇豆花叶病毒 1（Vigna unguiculata）中的 VuSOC1。我们使用定量实时 PCR 研究了 GmGAL1 的表达水平，结果表明 GmGAL1 受昼夜节律机制调节，其表达呈 24 小时周期振荡。GmGAL1 的表达水平在不同的组织/器官和发育阶段波动。考虑到其表达可以在整个生命周期的不同组织中检测到，并且其在拟南芥原生质体中的蛋白质定位于细胞质中，我们提出 GmGAL1 可能是大豆发育中具有多功能的基因。在长日照条件下，GmGAL1 在拟南芥中的异位表达增强了开花，并且部分挽救了 soc1 晚开花型。
