Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
Z Naturforsch C J Biosci. 2011 Nov-Dec;66(11-12):581-7. doi: 10.1515/znc-2011-11-1207.
We have studied the effects of cleistanthin A and cleistanthin B, phytoconstituents isolated from the leaves of Cleistanthus collinus Roxb. (Euphorbiaceae), on blood pressure, electrocardiogram, and barium chloride-induced arrhythmia in Wistar rats. The two compounds were isolated by column chromatography and their identity was confirmed spectroscopically. A healthy, male Wistar rat was used to record the invasive blood pressure and electrocardiograph. The antiarrhythmic effects of cleistanthins A and B were studied using the barium chloride model. Both cleistanthin A and cleistanthin B showed a dose-dependent hypotensive effect. Both compounds reduced the mean blood pressure significantly although the dose required for the effect was higher in the case of cleistanthin B. In the electrocardiogram, cleistanthins A and B significantly altered the electrical activity of the heart, the changes were transient and of no further consequence. Intravenous injection of 64 microg or more of cleistanthins A and B caused a sudden respiratory depression without affecting the electrocardiogram. Cleistanthins A and B did not display any antiarrhythmic effect against barium chloride-induced arrhythmia. In conclusion, both cleistanthin A and cleistanthin B exert a hypotensive effect and have no antiarrhythmic effect against barium chloride-induced arrhythmia in Wistar rats.
我们研究了从 Cleistanthus collinus Roxb.（大戟科）叶片中分离得到的植物成分 cleistanthin A 和 cleistanthin B 对 Wistar 大鼠血压、心电图和氯化钡诱导心律失常的影响。这两种化合物通过柱色谱法分离，并通过光谱学确认其身份。使用雄性 Wistar 大鼠记录有创血压和心电图。使用氯化钡模型研究 cleistanthins A 和 B 的抗心律失常作用。Cleistanthin A 和 cleistanthin B 均表现出剂量依赖性降压作用。尽管 cleistanthin B 所需的剂量更高，但两种化合物均能显著降低平均血压。在心电图中，cleistanthins A 和 B 显著改变了心脏的电活动，这些变化是短暂的，没有进一步的后果。静脉注射 64μg 或更多的 cleistanthins A 和 B 会导致突然的呼吸抑制，而不会影响心电图。Cleistanthins A 和 B 对氯化钡诱导的心律失常没有显示出任何抗心律失常作用。总之，cleistanthin A 和 cleistanthin B 对 Wistar 大鼠均具有降压作用，对氯化钡诱导的心律失常没有抗心律失常作用。
