Parasuraman S, Raveendran R
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
J Young Pharm. 2012 Apr;4(2):73-7. doi: 10.4103/0975-1483.96616.
To study the diuretic effects of cleistanthin A and cleistanthin B, phytoconstituents were isolated from the leaves of Cleistanthus collinus in Wistar rats. The in vivo diuretic effects of cleistanthins A and B were determined according to the Lipschitz test. Prior to the experiment, the animals were fasted for 5 h and placed individually in metabolic cages. Cleistanthins A and B (12.5, 25, and 50 mg/kg) and furosemide (5 mg/kg) were suspended in 0.5% w/v carboxymethyl cellulose and administered orally. The urine was collected up to 5 h after administration and subsequently up to 24 h after administration. The acidity and urine volume were measured immediately. The urinary sodium and potassium levels were determined using a flame photometer, and the chloride level was determined by argentometric titration. The diuretic index and diuretic activity were calculated mathematically. While cleistanthins A and B showed a diuretic index of more than one, the diuretic activity of these compounds was less than one, indicating inferior activity compared with furosemide. Both cleistanthin A and B produced a significant increase in the urine volume and alterations in urinary electrolyte levels. However, the effect of the compounds was not dose dependent. Cleistanthin A and cleistanthin B exert diuretic effects in male Wistar rats without affecting the urinary acidity.
为研究 cleistanthin A 和 cleistanthin B 的利尿作用，从 Wistar 大鼠的棒柄花（Cleistanthus collinus）叶片中分离出植物成分。根据 Lipschitz 试验测定 cleistanthins A 和 B 的体内利尿作用。实验前，动物禁食 5 小时，然后单独置于代谢笼中。将 cleistanthins A 和 B（12.5、25 和 50 mg/kg）以及呋塞米（5 mg/kg）悬浮于 0.5% w/v 羧甲基纤维素中并口服给药。给药后 5 小时内收集尿液，随后在给药后 24 小时内收集尿液。立即测量尿液酸度和尿量。使用火焰光度计测定尿钠和钾水平，通过银量滴定法测定氯水平。通过数学计算得出利尿指数和利尿活性。虽然 cleistanthins A 和 B 的利尿指数大于 1，但这些化合物的利尿活性小于 1，表明其活性低于呋塞米。cleistanthin A 和 cleistanthin B 均使尿量显著增加，并使尿电解质水平发生改变。然而，这些化合物的作用不呈剂量依赖性。cleistanthin A 和 cleistanthin B 在雄性 Wistar 大鼠中发挥利尿作用，且不影响尿液酸度。
