在正常和肿瘤动物模型中利用磁共振成像研究A千金藤素和B千金藤素的生物分布特性。

Biodistribution properties of cleistanthin A and cleistanthin B using magnetic resonance imaging in a normal and tumoric animal model.

作者信息

Parasuraman Subramani, Raveendran Ramasamy, Ardestani Mehdi Shafiee, Ananthakrishnan Ramesh, Jabbari-Arabzadeh Ali, Alavidjeh Mohammad Shafiee, Aghasadeghi Mohammad Reza, Elangovan Sundararajan, Dhanapathi Halanaik

机构信息

Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.

出版信息

Pharmacogn Mag. 2012 Apr;8(30):129-34. doi: 10.4103/0973-1296.96559.

DOI:10.4103/0973-1296.96559

PMID:22701286

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3371434/

Abstract

AIM

To determine the biodistribution properties of cleistanthin A and cleistanthin B in rodents using magnetic resonance imaging (MRI).

MATERIALS AND METHODS

Cleistanthins A and B, constituents of Cleistanthus collinus Roxb., were labelled with gadolinium (Gd(3+)) directly and injected into normal and tumoric nude mice. The tissue signal intensity was measured using MRI to perform a noninvasive kinetic assay. Wistar rats were used for determination of the grayscale intensity to observe the distribution patterns of of cleistanthins A and B.

RESULTS

Cleistanthin A is kinetically more attractive to the gastrointestinal tract than is cleistanthin B, which gets accumulated in muscular tissues of mice in greater concentrations compared with cleistanthin A. Cleistanthin B but not cleistanthin A showed tumoric affinity and exhibited a tumor kinetic attraction in tumoric mice. In rats, cleistanthin A showed greater grayscale intensities in the brain, liver, and skeletal muscles in immediate post contrast MRI images, whereas the gadolinium tagged cleistanthin B showed higher grayscale intensities in the cardiac muscle and skeletal muscles in delayed post contrast MRI images.

CONCLUSIONS

Cleistanthin A is more pharmacokinetically attractive to the gastrointestinal tract than cleistanthin B.

摘要

目的

利用磁共振成像（MRI）确定啮齿动物体内铁屎米酮A和铁屎米酮B的生物分布特性。

材料与方法

将铁屎米酮A和铁屎米酮B（铁屎米属植物的成分）直接用钆（Gd(3+)）标记，并注射到正常和患肿瘤的裸鼠体内。使用MRI测量组织信号强度以进行非侵入性动力学分析。使用Wistar大鼠测定灰度强度，以观察铁屎米酮A和铁屎米酮B的分布模式。

结果

与铁屎米酮B相比，铁屎米酮A在动力学上对胃肠道更具吸引力，而铁屎米酮B在小鼠肌肉组织中的积累浓度高于铁屎米酮A。铁屎米酮B而非铁屎米酮A显示出对肿瘤的亲和力，并在患肿瘤的小鼠中表现出肿瘤动力学吸引力。在大鼠中，在造影后即刻的MRI图像中，铁屎米酮A在脑、肝和骨骼肌中显示出更高的灰度强度，而钆标记的铁屎米酮B在造影后延迟的MRI图像中在心肌和骨骼肌中显示出更高的灰度强度。