视紫红质在天然膜中与细胞质螺旋 8 接触形成二聚体。
Rhodopsin forms a dimer with cytoplasmic helix 8 contacts in native membranes.
机构信息
Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065, United States.
出版信息
Biochemistry. 2012 Mar 6;51(9):1819-21. doi: 10.1021/bi3001598. Epub 2012 Feb 27.
Abstract
G protein-coupled receptors form dimers and higher-order oligomers in membranes, but the precise mode of receptor-receptor interaction remains unknown. To probe the intradimeric proximity of helix 8 (H8), we conducted chemical cross-linking of endogenous cysteines in rhodopsin in disk membranes. We identified a Cys316-Cys316 cross-link using partial proteolysis and liquid chromatography with mass spectrometry. These results show that a symmetric dimer interface mediated by H1 and H8 contacts is present in native membranes.
摘要
G 蛋白偶联受体在膜中形成二聚体和更高阶的寡聚体，但受体-受体相互作用的确切模式仍不清楚。为了探测螺旋 8（H8）的二聚体内接近程度，我们在盘状膜中的视紫红质内进行了内源性半胱氨酸的化学交联。我们使用部分蛋白水解和液相色谱-质谱联用鉴定了一个 Cys316-Cys316 交联。这些结果表明，在天然膜中存在由 H1 和 H8 接触介导的对称二聚体界面。
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