Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Sci Rep. 2011;1:139. doi: 10.1038/srep00139. Epub 2011 Nov 7.
Since plasma lipoproteins contain both protein and phospholipid components, either may be involved in processes such as atherosclerosis. In this study the identification of plasma lipoprotein-associated phospholipids, which is essential for understanding these processes at the molecular level, are performed. LC-ESI/MS, LC-ESI-MS/MS and High Performance Thin Layer Chromatography (HPTLC) analysis of different lipoprotein fractions collected from pooled plasma revealed the presence of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and sphingomyeline (SM) only on lipoproteins and phosphatidylcholine (PC), Lyso-PC on both lipoproteins and plasma lipoprotein free fraction (PLFF). Cardiolipin, phosphatidylglycerol (PG) and Phosphatidylserine (PS) were observed neither in the lipoprotein fractions nor in PLFF. All three approaches led to the same results regarding phospholipids occurrence in plasma lipoproteins and PLFF. A high abundancy of PE and SM was observed in VLDL and LDL fractions respectively. This study provides for the first time the knowledge about the phospholipid composition of all defined plasma lipoproteins.
由于血浆脂蛋白既含有蛋白质又含有磷脂成分，因此它们都可能参与动脉粥样硬化等过程。在本研究中，对与血浆脂蛋白相关的磷脂进行了鉴定，这对于在分子水平上理解这些过程是必不可少的。从混合血浆中收集的不同脂蛋白级分的 LC-ESI/MS、LC-ESI-MS/MS 和高效薄层色谱 (HPTLC) 分析表明，只有脂蛋白上存在磷脂酰乙醇胺 (PE)、磷脂酰肌醇 (PI) 和神经鞘磷脂 (SM)，而在脂蛋白和血浆脂蛋白游离部分 (PLFF) 上则存在磷脂酰胆碱 (PC)、溶血磷脂酰胆碱 (Lyso-PC)。心磷脂、磷脂酰甘油 (PG) 和磷脂酰丝氨酸 (PS) 既不在脂蛋白级分中也不在 PLFF 中观察到。这三种方法在关于磷脂在血浆脂蛋白和 PLFF 中的存在方面得出了相同的结果。VLDL 和 LDL 级分中分别观察到 PE 和 SM 的高丰度。本研究首次提供了关于所有定义的血浆脂蛋白中磷脂组成的知识。
