Department of Bioorganic Chemistry, Max-Planck Institute for Chemical Ecology, Jena, Germany.
FEMS Microbiol Ecol. 2012 Jun;80(3):659-70. doi: 10.1111/j.1574-6941.2012.01335.x. Epub 2012 Mar 12.
Although hematophagous black flies are well-known socioeconomic pests and vectors of disease agents, their associated bacteria are poorly known. A systematic analysis of the bacterial community associated with freshly emerged adult black flies of four North American species, using cultivation-independent molecular techniques, revealed 75 nonsingleton bacterial phylotypes. Although 17 cosmopolitan phylotypes were shared among host species, each fly species had a distinct bacterial profile. The bacterial composition, however, did not correlate strongly with the host phylogeny but differed between male and female flies of the same species from the same habitat, demonstrating that a group of insects have a gender-dependent bacterial community. In general, female flies harbor a less diverse bacterial community than do males. The anatomical locations of selected bacteria were revealed using fluorescence in situ hybridization. Understanding the physiological function of the associated bacterial community could provide clues for developing novel pest-management strategies.
尽管吸血性黑蝇是众所周知的社会经济害虫和疾病媒介，但它们相关的细菌却鲜为人知。采用非培养的分子技术对刚羽化的 4 种北美黑蝇进行了系统分析，发现了 75 个非单型细菌类群。尽管 17 个世界性类群在宿主物种中共享，但每种黑蝇都有独特的细菌谱。然而，细菌组成与宿主进化关系并不密切，而与同一栖息地同种雌雄蝇之间存在差异，这表明一组昆虫具有性别依赖的细菌群落。一般来说，雌性蝇比雄性蝇携带的细菌群落多样性更低。利用荧光原位杂交揭示了选定细菌的解剖位置。了解相关细菌群落的生理功能可以为开发新的害虫管理策略提供线索。
