Seto F
Zoology Department, University of Oklahoma, Norman 73019.
Poult Sci. 1990 Jul;69(7):1103-9. doi: 10.3382/ps.0691103.
The immune responsiveness of immunocyte precursors, obtained from peripheral blood of 4- to 6-wk-old unprimed chickens and assayed with the cell transfer model, was compared with that of neonatal chicks. Little if any splenic plaque-forming cell (PFC) production was observed in the spleens of embryonic cell transfer hosts following a single antigen exposure to weak or moderate dosages of mammalian erythrocytes. However, modest to high PFC formation occurred consistently in the hosts following a second exposure to the same antigen when given 3 days later. Although a single immunization failed to elicit PFC formation in embryo hosts, a single injection of a wide range of antigen dosages into 8-day-old chicks elicited PFC production consistently. When the double immunization protocol was used with 2- to 9-day-old neonatal chicks, the level of immune responses were indistinguishable from those elicited with a single antigen injection. The difference in the immune kinetics observed in embryo hosts may be related to the embryonic microenvironment, which is permissive, but may not be as immunosupportive as that of neonates.
从4至6周龄未致敏鸡的外周血中获取免疫细胞前体，并采用细胞转移模型进行检测，将其免疫反应性与新生雏鸡的进行比较。在胚胎细胞转移宿主的脾脏中，单次暴露于低剂量或中等剂量的哺乳动物红细胞抗原后，几乎未观察到脾空斑形成细胞（PFC）产生。然而，当在3天后再次暴露于相同抗原时，宿主中持续出现中等至高的PFC形成。虽然单次免疫未能在胚胎宿主中引发PFC形成，但向8日龄雏鸡单次注射多种抗原剂量均能持续引发PFC产生。当对2至9日龄的新生雏鸡采用双重免疫方案时，免疫反应水平与单次抗原注射引发的反应无法区分。在胚胎宿主中观察到的免疫动力学差异可能与胚胎微环境有关，胚胎微环境具有容许性，但可能不如新生雏鸡的微环境那样具有免疫支持性。
