Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States.
Langmuir. 2012 Jun 19;28(24):8834-44. doi: 10.1021/la3000944. Epub 2012 Mar 13.
The stability of gold nanorods (NRs) coated with amphiphilic ligands (ALs) was investigated. NRs coated with cetyltrimethylammonium bromide (CTAB) were ligand exchanged with polyoxyethylene [10] cetyl ether (Brij56), Oligofectamine (OF), and phosphatidylserine (PS). An aggregation index based on the longitudinal surface plasmon resonance peak broadening was used to measure stability of the NR-ALs under different conditions including the number of washes, pH, ionic concentration, and temperature. The aggregation index was also used to measure the stability of the NR-ALs under ultrafast laser irradiation and in the presence of proteins commonly used in cell culture. Differences in NR-AL stability were found, which were due to differences in the physical and chemical properties of the ALs. Apart from the charge on the AL headgroup, we suggest the Gibbs free energy of passivation (ΔG(p)) and enthalpy of passivation (ΔH(p)) of the AL could potentially aid in the selection of amphiphiles that can effectively passivate NRs for stability and optimize their properties and desired biological impact.
研究了具有两亲配体（ALs）的金纳米棒（NRs）的稳定性。用聚氧乙烯[10]鲸蜡醚（Brij56）、Oligofectamine（OF）和磷脂酰丝氨酸（PS）对十六烷基三甲基溴化铵（CTAB）包覆的 NRs 进行配体交换。基于纵向表面等离子体共振峰展宽的聚集指数用于测量 NR-AL 在不同条件下的稳定性，包括洗涤次数、pH 值、离子浓度和温度。该聚集指数还用于测量 NR-AL 在超快激光辐射下以及在细胞培养中常用的蛋白质存在下的稳定性。发现 NR-AL 的稳定性存在差异，这是由于 AL 的物理和化学性质的差异所致。除了 AL 头基的电荷外，我们还建议 AL 的钝化吉布斯自由能（ΔG(p)）和焓（ΔH(p)）可能有助于选择能够有效钝化 NRs 以实现稳定性的两亲分子，并优化它们的性质和所需的生物学影响。
