Department of Biological Engineering and ‡Department of Mechanical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
ACS Nano. 2014 May 27;8(5):4608-20. doi: 10.1021/nn5002886. Epub 2014 Apr 23.
We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. NRs coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphatidylserine (PS). Protein coronas from equine serum (ES) were formed on these NR-ALs, and their colloidal stability, as well as cell uptake, proliferation, oxidative stress, and gene expression, were examined. We find that the protein corona that forms and its colloidal stability are affected by AL type and that the cellular response to these NR-AL-coronas (NR-AL-ES) is both ligand and corona dependent. We also find that the presence of common cell culture supplement penicillin/streptomycin can impact the colloidal stability and cellular response of NR-AL and NR-AL-ES, showing that the cell response is not necessarily inert to pen/strep when in the presence of nanoparticles. Although the protein corona is what the cells see, the underlying surface ligands evidently play an important role in shaping and defining the physical characteristics of the corona, which ultimately impacts the cellular response. Further, the results of this study suggest that the cellular behavior toward NR-AL is mediated by not only the type of AL and the protein corona it forms but also its resulting colloidal stability and interaction with cell culture supplements.
我们探究了四种不同类型的两亲配体（ALs）如何影响金纳米棒（NRs）上蛋白质冠的形成及其对细胞反应的影响。用十六烷基三甲基溴化铵包覆的 NR 用聚氧乙烯[10]鲸蜡醚、寡聚阳离子脂质体和磷脂酰丝氨酸（PS）进行配体交换。在这些 NR-AL 上形成了来自马血清（ES）的蛋白质冠，并检查了它们的胶体稳定性以及细胞摄取、增殖、氧化应激和基因表达。我们发现，形成的蛋白质冠及其胶体稳定性受到 AL 类型的影响，并且细胞对这些 NR-AL-冠（NR-AL-ES）的反应既依赖于配体又依赖于冠。我们还发现，常见的细胞培养补充剂青霉素/链霉素的存在会影响 NR-AL 和 NR-AL-ES 的胶体稳定性和细胞反应，表明当存在纳米粒子时，细胞反应对青霉素/链霉素不一定是惰性的。尽管蛋白质冠是细胞所看到的，但潜在的表面配体显然在塑造和定义冠的物理特性方面起着重要作用，这最终会影响细胞反应。此外，这项研究的结果表明，NR-AL 细胞行为不仅受到 AL 类型和它形成的蛋白质冠的影响，还受到其胶体稳定性及其与细胞培养补充剂的相互作用的影响。
