Dipartimento di Scienze Farmaceutiche, Università di Modena e Reggio Emilia, Via Campi 183, 41125 Modena, Italy.
J Agric Food Chem. 2012 Mar 21;60(11):2852-62. doi: 10.1021/jf205179d. Epub 2012 Mar 12.
The composition and antioxidant activity of Italian poplar propolis obtained using three harvesting methods and extracted with different solvents were evaluated. Waxes, balsams, and resins contents were determined. Flavones and flavonols, flavanones and dihydroflavonols, and total phenolics were also analyzed. To characterize the phenolic composition, the presence of 15 compounds was verified through HPLC-MS/MS. The antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl radical and reducing power assays. The ability of propolis to inhibit lipid oxidation was monitored by analyzing hydroperoxide and TBARS formation in lipids incorporated into an oil-in-water (O/W) emulsion. Acetone shows the highest extraction capacity. Wedge propolis has the highest concentration of active phenolic compounds (TP = 359.1 ± 16.3 GAEs/g; TFF = 5.83 ± 0.42%; TFD = 7.34 ± 1.8%) and seems to be the most promising for obtaining high-value propolis more suitable to prepare high-quality dietary supplements (TBARS = 0.012 ± 0.009 mmol std/g; RP = 0.77 ± 0.07 TEs/g).
采用三种采收方法和三种不同溶剂提取的意大利白杨蜂胶的组成和抗氧化活性进行了评价。测定了蜂蜡、香树脂和树脂的含量。还分析了类黄酮和黄酮醇、黄烷酮和二氢黄酮醇以及总酚类物质。为了表征酚类成分，通过 HPLC-MS/MS 验证了 15 种化合物的存在。通过 1,1-二苯基-2-苦基肼自由基和还原能力测定评估了抗氧化活性。通过分析脂质中加入油包水（O/W）乳液中的过氧化物和 TBARS 形成来监测蜂胶抑制脂质氧化的能力。丙酮显示出最高的提取能力。楔形蜂胶具有最高浓度的活性酚类化合物（TP = 359.1 ± 16.3 GAEs/g；TFF = 5.83 ± 0.42%；TFD = 7.34 ± 1.8%），似乎是获得更适合制备高质量膳食补充剂的高价值蜂胶的最有前途的方法（TBARS = 0.012 ± 0.009 mmol std/g；RP = 0.77 ± 0.07 TEs/g）。
