Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq.
Food Chem Toxicol. 2011 Sep;49(9):2415-21. doi: 10.1016/j.fct.2011.06.060. Epub 2011 Jun 24.
Propolis samples, collected from different geographical locations in Iraq (Baghdad, Dahuk, Mosul and Salah ad-Din), were analyzed and assessed for their anti-oxidant activity. Concentrations of phenolic compounds (flavonoids, phenolic acids and their esters) in propolis were estimated using high performance liquid chromatography coupled to electrospray mass spectrometry. Thirty-eight different compounds were identified and 33 of them were polyphenols. Other compounds were tentatively identified as clerodane diterpenoids, and one was considered unknown. Semi-quantitative measurements showed that phenolic acids and their esters were the predominant constituents in propolis extracts, followed by flavones and flavonols, and then flavanones and dihydroflavonols. Propolis samples were further spectrophotometrically characterized using the Folin-Ciocalteu reagent for the determination of total phenolic compounds. The free radical scavenging activities of propolis samples were also evaluated by using the 2,2-diphenyl-1-picrylhydrazyl assay. The results revealed that propolis extracts exhibited strong free radical scavenging activity.
从伊拉克不同地理位置（巴格达、达胡克、摩苏尔和萨拉丁省）采集的蜂胶样品进行了分析，并评估了它们的抗氧化活性。使用高效液相色谱法-电喷雾质谱联用技术测定了蜂胶中酚类化合物（类黄酮、酚酸及其酯）的浓度。鉴定出 38 种不同的化合物，其中 33 种为多酚。其他化合物被初步鉴定为 clerodane 二萜类，一种被认为是未知的。半定量测量表明，在蜂胶提取物中，酚酸及其酯是主要成分，其次是黄酮类和黄酮醇类，然后是黄烷酮类和二氢黄酮醇类。进一步使用 Folin-Ciocalteu 试剂分光光度法测定了总酚类化合物，并用 2,2-二苯基-1-苦基肼法评价了蜂胶样品的自由基清除活性。结果表明，蜂胶提取物具有很强的自由基清除活性。
