Department of Radiation Medicine, Faculty of Naval Medicine, 2nd Military Medical University, Shanghai, PR China.
Med Sci Monit. 2012 Mar;18(3):BR89-94. doi: 10.12659/msm.882513.
Recent studies show that molecular hydrogen (dihydrogen, H2) has potential as an effective and safe radioprotective agent through reducing oxidative stress. The aim of this study was to investigate whether H2 is able to protect spermatogenesis and hematopoiesis from radiation-induced injuries.
MATERIAL/METHODS: H2 was dissolved in physiological saline using an apparatus produced by our department. -60Co-gamma rays in the irradiation centre were used for irradiation. Spermatid head counts and histological analysis were used to evaluate spermatogenesis. Endogenous hematopoietic spleen colony formation (endoCFUs), bone marrow nucleated cells (BMNC) and peripheral blood (PB) leukocytes were used to evaluate hemopoiesis.
This study demonstrates that treating mice with H2 before ionizing radiation (IR) can increase the spermatid head count and protect seminiferous epithelium from IR. This study also demonstrates that H2 could significantly increase the number of endoCFUs, BMNC and PB leukocyte.
This study suggests that hydrogen-rich saline could partially protect spermatogenesis and hematopoiesis in irradiated mice.
最近的研究表明，通过减少氧化应激，分子氢（氢气，H2）有作为一种有效且安全的辐射防护剂的潜力。本研究旨在探讨 H2 是否能够保护生精和造血免受辐射损伤。
材料/方法：使用本部门生产的装置将 H2 溶解在生理盐水中。辐照中心的 -60Co-γ射线用于照射。精子头部计数和组织学分析用于评估生精功能。内源性造血脾集落形成（endoCFUs）、骨髓有核细胞（BMNC）和外周血（PB）白细胞用于评估造血功能。
本研究表明，在电离辐射（IR）前用 H2 处理小鼠可以增加精子头部计数并保护曲细精管免受 IR。本研究还表明，H2 可显著增加 endoCFUs、BMNC 和 PB 白细胞的数量。
本研究表明富氢生理盐水可部分保护辐照小鼠的生精和造血功能。
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