Applied Science and Technology Department, Politecnico di Torino, Torino, Italy.
J Appl Biomater Funct Mater. 2012 Jun 26;10(1):12-21. doi: 10.5301/JABFM.2012.9270.
Coupling the potential for bone regeneration and the ability for in situ controlled drug release in a single device is a challenging field of research in bone tissue engineering; in an attempt to pursue this aim, mesoporous bioactive glass (MBG) membranes belonging to the SiO2-P2O5-CaO ternary system were produced and characterized.
The glass was synthesized via a sol-gel route coupled with an evaporation-induced self-assembly process by using a non-ionic block co-polymer as a mesostructure former. MBG structure and morphology, as well as mesopores size and shape, were investigated by x-ray diffraction, transmission electron microscopy, and N2 adsorption-desorption measurements. In vitro bioactivity was investigated by soaking MBG membranes in simulated body fluid (SBF) for different time frames. Ibuprofen was encapsulated into MBG pores and drug release kinetics in SBF were assessed. Biological tests by using SAOS-2 cells were performed to assess the material cytocompatibility.
The material revealed significant ability to induce hydroxyapatite formation on its surface (bioactivity). Drug release kinetics in SBF are very similar to those obtained for mesoporous silica having mesopore size comparable to that of the prepared MBG (∼5 nm). No evidence of cell viability depression was detected during in vitro culture, which demonstrates the good biological compatibility of the material.
The easiness of tailoring and shaping, the highly bioactive and biocompatible behavior, and the drug uptake/release ability of the prepared materials may suggest their use as "smart" multifunctional grafts for bone reconstructive surgery.
将骨再生的潜力与原位控制药物释放的能力结合在单个装置中，是骨组织工程中具有挑战性的研究领域；为了追求这一目标，合成了属于 SiO2-P2O5-CaO 三元体系的介孔生物活性玻璃（MBG）膜，并对其进行了表征。
采用溶胶-凝胶法与蒸发诱导自组装法相结合，使用非离子嵌段共聚物作为介孔结构前体来合成玻璃。通过 X 射线衍射、透射电子显微镜和 N2 吸附-脱附测量研究了 MBG 的结构和形态以及介孔的大小和形状。通过将 MBG 膜浸泡在模拟体液（SBF）中不同时间来研究其体外生物活性。将布洛芬包封到 MBG 孔中，并评估了在 SBF 中的药物释放动力学。通过使用 SAOS-2 细胞进行生物学测试来评估材料的细胞相容性。
该材料在其表面具有显著的诱导羟基磷灰石形成的能力（生物活性）。在 SBF 中的药物释放动力学与具有与制备的 MBG 相似的介孔尺寸（约 5nm）的介孔硅相似。在体外培养过程中未发现细胞活力降低的证据，这证明了材料的良好生物相容性。
所制备材料的易于定制和成型、高度的生物活性和生物相容性以及药物摄取/释放能力表明它们可用作骨重建手术的“智能”多功能移植物。
