从采集于巴拿马的与八放珊瑚相关的芽孢杆菌属细菌中分离出的杆菌肌动蛋白A的抗疟活性。
Antiplasmodial activity of bacilosarcin A isolated from the octocoral-associated bacterium Bacillus sp. collected in Panama.
作者信息
Boya Cristopher A, Herrera Liuris, Guzman Hector M, Gutierrez Marcelino
机构信息
Center for Drug Discovery, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge, Republic of Panama.
出版信息
J Pharm Bioallied Sci. 2012 Jan;4(1):66-9. doi: 10.4103/0975-7406.92739.
AIM
This study was designed for isolating and characterizing antiplasmodial compounds from marine octocoral-associated bacteria.
MATERIALS AND METHODS
The organic extract of the Bacillus sp. was subjected to purification using several chromatography techniques guided by bioassays to yield three isocoumarin derivatives (1-3). Chemical structures of the compounds were elucidated on the basis of HRMS spectra and NMR spectroscopy. The antiplasmodial activity of the isolated compounds was evaluated in vitro against the chloroquine-resistant Plasmodium falciparum strain W2.
RESULTS
Isolated compounds were identified as bacilosarcin A (1), AI77-F (2), and AI77-H (3). Bacilosarcin A (1) displayed a low micromolar activity (IC(50) = 2.2 μM) against P. falciparum while compounds 2 and 3 showed no activity.
CONCLUSIONS
Bacilosarcin A was found to be responsible for the antiplasmodial activity observed in the crude extract obtained from the Bacillus sp.
目的
本研究旨在从与海洋八放珊瑚相关的细菌中分离和鉴定抗疟化合物。
材料与方法
芽孢杆菌属的有机提取物在生物测定的指导下，采用多种色谱技术进行纯化，得到三种异香豆素衍生物（1-3）。根据高分辨质谱光谱和核磁共振光谱确定化合物的化学结构。体外评估分离出的化合物对耐氯喹恶性疟原虫菌株W2的抗疟活性。
结果
分离出的化合物鉴定为芽孢杆菌素A（1）、AI77-F（2）和AI77-H（3）。芽孢杆菌素A（1）对恶性疟原虫表现出低微摩尔活性（IC(50)=2.2μM），而化合物2和3无活性。
结论
发现芽孢杆菌素A是从芽孢杆菌属获得的粗提物中观察到的抗疟活性的原因。
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