Boya Cristopher A, Herrera Liuris, Guzman Hector M, Gutierrez Marcelino
Center for Drug Discovery, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge, Republic of Panama.
J Pharm Bioallied Sci. 2012 Jan;4(1):66-9. doi: 10.4103/0975-7406.92739.
This study was designed for isolating and characterizing antiplasmodial compounds from marine octocoral-associated bacteria.
The organic extract of the Bacillus sp. was subjected to purification using several chromatography techniques guided by bioassays to yield three isocoumarin derivatives (1-3). Chemical structures of the compounds were elucidated on the basis of HRMS spectra and NMR spectroscopy. The antiplasmodial activity of the isolated compounds was evaluated in vitro against the chloroquine-resistant Plasmodium falciparum strain W2.
Isolated compounds were identified as bacilosarcin A (1), AI77-F (2), and AI77-H (3). Bacilosarcin A (1) displayed a low micromolar activity (IC(50) = 2.2 μM) against P. falciparum while compounds 2 and 3 showed no activity.
Bacilosarcin A was found to be responsible for the antiplasmodial activity observed in the crude extract obtained from the Bacillus sp.
本研究旨在从与海洋八放珊瑚相关的细菌中分离和鉴定抗疟化合物。
芽孢杆菌属的有机提取物在生物测定的指导下，采用多种色谱技术进行纯化，得到三种异香豆素衍生物（1-3）。根据高分辨质谱光谱和核磁共振光谱确定化合物的化学结构。体外评估分离出的化合物对耐氯喹恶性疟原虫菌株W2的抗疟活性。
分离出的化合物鉴定为芽孢杆菌素A（1）、AI77-F（2）和AI77-H（3）。芽孢杆菌素A（1）对恶性疟原虫表现出低微摩尔活性（IC(50)=2.2μM），而化合物2和3无活性。
发现芽孢杆菌素A是从芽孢杆菌属获得的粗提物中观察到的抗疟活性的原因。
