Zhong Ling-Yun, Zhang Ying, Huo Hui-Jun, Gong Qian-Feng
Pharmacy College of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Zhong Yao Cai. 2011 Oct;34(10):1508-11.
To discuss the processing mechanism of Rhizoma Polygonati (RP) through studying the correlation between the change of composition and pharmacological function in raw and processed RP.
The extraction of petroleum ether, methylene dichloride, ethyl acetate and 1-butanol of the raw and processed RP were compared by HPLC. The compounds changed in processed RP in the methylene dichloride extraction were further identified with reference substances. The immune function of methylene dichloride extraction of raw and processed RP were compared.
The changed compound in concentration was determined to be 5-Hydroxymethylfurfural. After processed, the concentration of 5-Hydroxymethylfurfural sharply increased. The carbon clearance index (P < 0.01) and coefficient of phagocytosis (P < 0.05) were increased remarkably by processed RP comparing to those of the normal saline and raw RP.
The increase of immune function of processed RP may be related to increasing of concentration of 5-Hydroxymethylfurfural. The results provide a better understanding of RP processing.
通过研究生熟黄精成分变化与药理作用的相关性，探讨黄精的炮制机制。
采用高效液相色谱法比较生熟黄精石油醚、二氯甲烷、乙酸乙酯和正丁醇提取物。用对照品进一步鉴定二氯甲烷提取物中炮制后黄精变化的化合物。比较生熟黄精二氯甲烷提取物的免疫功能。
确定浓度变化的化合物为5-羟甲基糠醛。炮制后，5-羟甲基糠醛的浓度急剧增加。与生理盐水和生黄精相比，炮制黄精显著提高了碳廓清指数（P<0.01）和吞噬系数（P<0.05）。
炮制黄精免疫功能的增强可能与5-羟甲基糠醛浓度的增加有关。该结果为黄精炮制提供了更好的认识。
